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GeneBLAzer™ P2RY2-NFAT-bla CHO-K1 Cells

Catalog number:  K1722

Related applications:

Pharma & Biopharma

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Description

GeneBLAzer® P2RY2-NFAT-bla CHO-K1 cells contain the human Purinergic receptor P2, G protein-coupled, 2 (P2RY2) stably integrated into the CellSensor® NFAT-bla CHO-K1 cell line. CellSensor® NFAT-bla CHO-K1 cells (Cat. no. K1534) contain a beta-lactamase reporter gene under control of the Nuclear Factor of Activated T-cells (NFAT) response element.

The GeneBLAzer® P2RY2-NFAT-bla CHO-K1 cells are functionally validated for Z' and EC50 concentrations of ATP. In addition, GeneBLAzer® P2RY2-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.

P2RY2 expression has been identified in smooth muscle, skeletal muscle, heart, spleen, lymphocytes, macrophages, bone marrow, lung, intestine, placenta, brain, kidney, and liver. P2RY2 has been implicated in the induction of chloride secretion in airway epithelial tissue, increases in coronary blood flow by vasodilation, and epidermal homeostasis.

For Academic pricing please login or contact us at discoverysciences@invitrogen.com.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Accession Number: BC028135.1
Assay Entry: Cell-based beta lactamase reporter gene
Cell Line: CHO-K1
Cell State: Dividing Cells
Druggable Target: GPCRs
Gene Symbol: P2RY2
Population Status: Single Cell Clone Origin
Product Line: GeneBLAzer®
Reporter Gene: BLA (Beta-Lactamase)
System Type: GeneBLAzer™
Target Entry: P2RY2
Technique: FRET
Detection Method: Fluorescent

Contents & storage

Contents:
K1722 GeneBLAzer® P2RY2-NFAT-bla CHO-K1 cells
(LiveBLAzer™-FRET B⁄G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
Includes:
• GeneBLAzer® P2RY2-NFAT-bla CHO-K1 cells
• GeneBLAzer® P2RY2-NFAT-bla CHO-K1 Assay Protocol
• Certificate of Analysis

Storage:
The GeneBLAzer® P2RY2-NFAT-bla CHO-K1 cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.