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Thermo Scientific™

GeneBLAzer™ M5-NFAT-bla CHO-K1 Cells

Catalog number: K1728
Thermo Scientific™

GeneBLAzer™ M5-NFAT-bla CHO-K1 Cells

Catalog number: K1728
Catalog Number
K1728
Unit Size
2 x 106 cells
Price (USD)
8,140.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
K17282 x 106 cells
8,140.00
Your Price:
***
Product Overview
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GeneBLAzer® M5-NFAT-bla CHO-K1 cells contain the human Acetylcholine (Muscarinic) Receptor 5 (M5) stably integrated into the CellSensor® NFAT-bla CHO-K1 cell line. CellSensor® NFAT-bla CHO-K1 cells (Cat. no. K1534) contain a beta-lactamase reporter gene under control of the Nuclear Factor of Activated T-cells (NFAT) response element.

The GeneBLAzer® M5-NFAT-bla CHO-K1 cells are functionally validated for Z' and EC50 concentrations of Carbachol. In addition, GeneBLAzer® M5-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.

Muscarinic acetylcholine receptor subtype 5 (M5) has an unknown physiological relevance primarily because of low expression levels and the lack of M5 receptor-selective ligands. However, knockout studies have suggested that M5 is involved in modulation of central dopamine function and the tone of cerebral blood vessels.

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Specifications

Accession Number
BC041805.1
Assay Entry
Cell-based beta lactamase reporter gene
Cell State
Dividing Cells
Detection Method
Fluorescent
For Use With (Application)
FRET
Gene Symbol
CHRM5
Population Status
Single Cell Clone Origin
Product Line
GeneBLAzer™
Reporter Gene
BLA (Beta-Lactamase)
Shipping Condition
Room Temperature
Target Entry
CHRM5, M5
Cell Line
CHO-K1
System Type
GeneBLAzer™
Druggable Target
GPCRs

Contents & Storage

Contents:
K1728 GeneBLAzer™ M5-NFAT-bla CHO-K1 cells
(LiveBLAzer™-FRET B⁄G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
Includes:
• GeneBLAzer™ M5-NFAT-bla CHO-K1 cells
• GeneBLAzer™ M5-NFAT-bla CHO-K1 Assay Protocol
• Certificate of Analysis

Storage:
The GeneBLAzer™ M5-NFAT-bla CHO-K1 cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.

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