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Thermo Scientific™

GeneBLAzer™ M1-NFAT-bla CHO-K1 Cells

Catalog number: K1735
Thermo Scientific™

GeneBLAzer™ M1-NFAT-bla CHO-K1 Cells

Catalog number: K1735
Catalog Number
K1735
Unit Size
2 x 106 cells
Price (USD)
8,140.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
K17352 x 106 cells
8,140.00
Your Price:
***
Product Overview
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GeneBLAzer® M1-NFAT-bla CHO-K1 cells contain the human Acetylcholine (muscarinic) subtype 1 receptor (M1) stably integrated into the CellSensor® NFAT-bla CHO-K1 cell line. CellSensor® NFAT-bla CHO-K1 cells (Cat. no. K1534) contain a beta-lactamase (bla) reporter gene under control of the Nuclear Factor of Activated T-cells (NFAT) response element.

M1-NFAT-bla CHO-K1 cells are functionally validated for Z'-factor and EC50 concentrations of carbachol. In addition, M1-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.

In the brain, M1 activation mediates 'slow' neuronal excitability. Cortical and hippocampal muscarinic receptors are thought to be important in the attentional aspects of cognition. The predominant receptor subtypes in these brain areas are M1, M3, and M4. Therefore, M1 is a potential target for cognition, Alzheimer's, dementia, and schizophrenia. Studies on knock-out mouse models of M1 are also beginning to reveal potential functions of the receptor. Additional information on the muscarinic receptors can be found in reviews.

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Specifications

Accession Number
NM_000738
Assay Entry
Cell-based beta lactamase reporter gene
Cell State
Dividing Cells
Detection Method
Fluorescent
For Use With (Application)
FRET
Gene Symbol
M1
Population Status
Single Cell Clone Origin
Product Line
GeneBLAzer™
Reporter Gene
BLA (Beta-Lactamase)
Target Entry
M1
Cell Line
CHO-K1
System Type
GeneBLAzer™
Druggable Target
GPCRs

Contents & Storage

Contents:
K1735 GeneBLAzer™ M1-NFAT-bla CHO-K1 cells
(LiveBLAzer™-FRET B⁄G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
Includes:
• GeneBLAzer™ M1-NFAT-bla CHO-K1 cells
• GeneBLAzer™ M1-NFAT-bla CHO-K1 Assay Protocol
• Certificate of Analysis

Storage:
The GeneBLAzer™ M1-NFAT-bla CHO-K1 cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.

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