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Thermo Scientific™

GeneBLAzer™ PTGDR-CRE-bla CHO-K1 Cells

Catalog number:  K1739

Related applications:

Pharma & Biopharma

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Description

GeneBLAzer™ PTGDR-CRE-bla CHO-K1 cells contain the human Prostanoid (PTGDR) Receptor stably integrated into the CellSensor™ CRE-bla CHO-K1 cell line. CellSensor™ CRE-bla CHO-K1 cells (Cat. no. K1535) contain a beta-lactamase reporter gene under control of the Cyclic AMP Response Element (CRE) response element.

The GeneBLAzer™ PTGDR-CRE-bla CHO-K1cells are functionally validated for Z' and EC50 concentrations of Prostaglandin D2. In addition, GeneBLAzer™ PTGDR-CRE-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.

The PTGDR receptor also known as DP, is a Gs-coupled receptor which activates adenylate cyclase. PTGDR receptors are located in vascular smooth muscle cells and blood platelets, and are also believed to be expressed in the brain, where they may be involved in the regulation of sleep. In addition, knockout studies in mice suggest that PTGDR may be involved in allergic and asthmatic responses. Potent agonists and antagonists for PTGDR have been identified.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Accession Number: NM_000953.2
Assay Entry: Cell-based beta lactamase reporter gene
Cell Line: CHO-K1
Cell State: Dividing Cells
Detection Method: Fluorescent
Druggable Target: GPCRs
Gene Symbol: PTGDR
Population Status: Single Cell Clone Origin
Product Line: GeneBLAzer™
Reporter Gene: BLA (Beta-Lactamase)
System Type: GeneBLAzer™
Target Entry: PTGDR
Technique: FRET

Contents & storage

Contents:
K1739 GeneBLAzer™ PTGDR-CRE-bla CHO-K1 cells
(LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
Includes:
• GeneBLAzer™ PTGDR-CRE-bla CHO-K1 cells
• GeneBLAzer™ PTGDR-CRE-bla CHO-K1 Assay Protocol
• Certificate of Analysis

Storage:
The GeneBLAzer™ PTGDR-CRE-bla CHO-K1 cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.