GeneBLAzer™ MC3R CRE-bla CHO-K1 cells contain the human Melanocortin Receptor 3 (MC3R) stably integrated into the CellSensor™ CRE-bla CHO-K1 cell line. CellSensor™ CRE-bla CHO-K1 cells (Cat. no. K1535) contain a beta-lactamase reporter gene under control of the Cyclic AMP Response Element (CRE) response element.

The GeneBLAzer™ MC3R CRE-bla CHO-K1 DA cells are functionally validated for Z' and EC₅₀ concentrations of NDP-α-MSH. In addition, GeneBLAzer™ MC3R CRE-bla CHO-K1 DA cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.

The melanocortin-3 receptor (MC3R) is primarily expressed in the hypothalamus, and plays an important role in the regulation of energy homeostasis. MC3R recognizes all of the melanocortin peptides, and notably binds - -MSH with greater affinity than the other melanocortin receptors. MC3R-deficient (MC3R-/-) mice demonstrate increased fat mass, higher feeding efficiency, and reduced lean body mass. To date only one mutation of MC3R has been identified to be associated with human obesity. Functional analysis of this mutation indicated a complete loss of agonist-mediated receptor activation.

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