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GeneBLAzer® C5aR1-Gα15-NFAT-bla CHO-K1 Cells

Catalog number: K1746

 Related applications: Target & Lead Identification & Validation

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Description

GeneBLAzer® C5aR1-Gα15-NFAT-bla CHO-K1 cells contain the human Complement Component 5a Receptor 1 (C5aR1) (Accession # NM_000710) stably integrated into the GeneBLAzer® Gα15-NFAT-bla CHO-K1 cell line. GeneBLAzer® Gα15-NFAT-bla CHO-K1 (Cat. No. K1537) contains a beta-lactamase reporter gene under control of a NFAT response element and a promiscuous G Protein, Gα15, stably integrated into CHO-K1 cells.

The GeneBLAzer® C5aR1-Gα15-NFAT-bla CHO-K1 cells are functionally validated for Z' and EC50 concentrations of C5a. In addition, GeneBLAzer® C5aR1-Gα15-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.

For Academic pricing please login or contact us at discoverysciences@invitrogen.com.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

System: GeneBLAzer®
Reporter: BLA (Beta-Lactamase)
Cell Line: CHO-K1
Technique: FRET
Cell State: Dividing Cells
Gene Symbol: C5aR1
Product Size: 2 x 106 cells
Accession Number: NM_001736.2
Detection Method: Fluorescent
Druggable Target: GPCRs
Population Status: Single Cell Clone Origin

Contents & storage

Contents:
K1746 GeneBLAzer® C5aR1-Gα15-NFAT-bla CHO-K1 cells
(LiveBLAzer™-FRET B⁄G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
Includes:
• GeneBLAzer® C5aR1-Gα15-NFAT-bla CHO-K1 cells
• GeneBLAzer® C5aR1-Gα15-NFAT-bla CHO-K1 Assay Protocol
• Certificate of Analysis

Storage:
The GeneBLAzer® C5aR1-Gα15-NFAT-bla CHO-K1 cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.

Documents

Manuals & protocols