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Thermo Scientific™

GeneBLAzer™ T-REx™-G2A-NFAT-bla FreeStyle™ 293F Cells

Catalog number: K1749
Thermo Scientific™

GeneBLAzer™ T-REx™-G2A-NFAT-bla FreeStyle™ 293F Cells

Catalog number: K1749
Catalog Number
K1749
Unit Size
2 x 106 cells
Price (USD)
8,140.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
K17492 x 106 cells
8,140.00
Your Price:
***
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GeneBLAzer® T-REx™ G2A-NFAT-bla Freestyle HEK 293F cells contain the human GPR132 (G2A), (Accession # NP_037477) stably integrated into the CellSensor® NFAT-bla Freestyle HEK 293F cell line. CellSensor® NFAT-bla Freestyle HEK 293F cells (Cat. no. K1725) contain a beta-lactamase reporter gene under control of the NFAT Response Element.

The GeneBLAzer® T-REx™ G2A-NFAT-bla Freestyle HEK 293F cells are functionally validated for Z' and EC50 concentrations of dDAVP. In addition, GeneBLAzer® GeneBLAzer® T-REx™ G2A-NFAT-bla Freestyle HEK 293F cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.

G2A is an orphan GPCR. Upon overexpession G2A is constitutively active. This is an assay for the orphan GPCR based upon constitutive activity. To use the assay to detect inverse agonists, high amounts of doxycycline should be added. To use the assay to detect agonists, low amounts of doxycycline should be added.

For Academic pricing please login or contact us at discoverysciences@invitrogen.com.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Accession Number
NM_013345
Assay Entry
Cell-based beta lactamase reporter gene
Cell State
Dividing Cells
Detection Method
Fluorescent
For Use With (Application)
FRET
Gene Symbol
G2A
Population Status
Single Cell Clone Origin
Product Line
GeneBLAzer™
Reporter Gene
BLA (Beta-Lactamase)
Target Entry
GPR132, G2A
Cell Line
FreeStyle™ 293-F Cells
System Type
GeneBLAzer™
Druggable Target
GPCRs

Contents & Storage

Contents:
K1749 GeneBLAzer™ TREx™-G2A-NFAT-bla Freestyle293F cells
(LiveBLAzer™-FRET B⁄G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
Includes:
• GeneBLAzer™ TREx™-G2A-NFAT-bla Freestyle293F cells
• GeneBLAzer™ TREx™-G2A-NFAT-bla Freestyle293F Assay Protocol
• Certificate of Analysis

Storage:
The GeneBLAzer™ TREx™-G2A-NFAT-bla Freestyle293F cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.

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