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The Tango™ GPR109A-bla U2OS cells contain the human Nicotinic Acid Receptor (GPR109A) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.
The Tango™ GPR109A-bla U2OS cells are functionally validated for Z' and EC50 concentrations of Nicotinic Acid. In addition, Tango™ GPR109A-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.
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For Research Use Only. Not for use in diagnostic procedures.
Cell-based beta-arrestin recruitment
For Use With (Application)
Single Cell Clone Origin
Contents & Storage
Contents: K1791 Tango™ GPR109A-bla U2OS cells (LiveBLAzer™-FRET B⁄G Loading Kit, Solution D and other materials are required separately; please refer to the protocol). Includes: • Tango™ GPR109A-bla U2OS cells • Tango™ GPR109A-bla U2OS Assay Protocol • Certificate of Analysis
Storage: The Tango™ GPR109A-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.