Invitrogen™

Easy-DNA™ gDNA Purification Kit

Catalog number: K180001
Invitrogen™

Easy-DNA™ gDNA Purification Kit

Catalog number: K180001
Catalog Number
K180001
also known as K1800-01
Unit Size
1 kit
Price (USD)
Price: 686.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
K180001
also known as K1800-01
1 kit
Price: 686.00
Your Price:
***
Product Overview
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Additional Information
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The Easy-DNA™ Kit is a fast and easy way to isolate high-quality, high-molecular weight genomic DNA (gDNA) from a wide range of cells and tissue types. Sample sizes can range from a single hair follicle up to 1 gram of mammalian tissue. Other samples tested include mouse tails, plant leaves, yeast, and E. coli cells. These samples yield high-quality DNA with an average size between 100 kb and 200 kb, which is suitable for PCR, DNA hybridization, genomic DNA library construction, and other applications. Advantages of the Easy-DNA™ Kit include:

Reliability—high-quality genomic DNA from large or small samples of cells, tissue, plant, yeast, E. coli, blood, and even hair follicles
Simplicity—for most sample types, only 4 steps, typically requiring less than 90 minutes are needed to isolate high-quality DNA
Versatility—one kit is supported by optimized protocols for a variety of cell and tissue types

Simple workflow, quality results
Optimized protocols for a variety of cell and tissue types are included in the instruction manual supplied with the kit. Generally, small-scale gDNA preparations are performed in microfuge tubes, in which samples are mixed with Lysis Solution and incubated at 65°C for 10 minutes. After extraction with chloroform (not included in kit), precipitation, and reconstitution of the DNA pellet, the preparation is complete. The kit contains a protein degradation reagent (for use with high-protein–containing samples such as connective tissue), mussel glycogen for use as a co-precipitant for dilute DNA samples, and an RNase solution.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format
Microfuge Tube
Isolation Technology
Organic Extraction
Sample Type
Bacteria, Cells, Fungi, Plant Samples, Tissue, Yeast
For Use With (Application)
Southern Blotting, PCR
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Product Line
Easy-DNA™
Product Type
gDNA Purification Kit
Target
Genomic DNA
Test Time
<90 min

Contents & Storage

The Easy-DNA™ Kit contains enough reagents for 15–200 samples, depending on sample size and type.

This kit contains:
• 55 ml Solution A (Lysis Solution)
• 25 ml Solution B (Precipitation Solution)
• 100 ml TE Buffer
• 750 µl Mussel Glycogen (2 mg/ml in sterile water)
• 750 µl RNase (2 mg/ml in sterile water)
• 750 µl Protein Degrader (5 mg/ml in sterile water)

Store kit at room temperature. For long-term storage, Mussel Glycogen, RNase, and Protein Degrader may be stored at -20°C.

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