The Tango™ TACR1-bla U2OS cells contain the human Tachykinin 1 (TACR1) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.
The Tango™ TACR1-bla U2OS cells are functionally validated for Z' and EC50 concentrations of (SAR9,MET(02)11)-Substance P. In addition, Tango™ TACR1-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.
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For Research Use Only. Not for use in diagnostic procedures.
Specifications
Accession Number
NM_001058
Assay Entry
Cell-based beta-arrestin recruitment
Cell State
Dividing Cells
Cytoplasmic Tail
Tail from GRPR GPCR
Detection Method
Fluorescent
For Use With (Application)
FRET
Gene Symbol
TACR1
Population Status
Single Cell Clone Origin
Product Line
GeneBLAzer™, Tango™
Readout
End Point
Reporter Gene
BLA (Beta-Lactamase)
Target Entry
TACR1
Cell Line
U2OS
System Type
Tango™
Druggable Target
GPCRs
Contents & Storage
Contents: K1801 Tango™ TACR1-bla U2OS cells (LiveBLAzer™-FRET B⁄G Loading Kit, Solution D and other materials are required separately; please refer to the protocol). Includes: • Tango™ TACR1-bla U2OS cells • Tango™ TACR1-bla U2OS Assay Protocol • Certificate of Analysis
Storage: The Tango™ TACR1-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.