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CellSensor® DBE-bla MDA-MB-468 Cell Line

Catalog number: K1814

 Related applications: Pharma & Biopharma | Target & Lead Identification & Validation

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The phosphatidylinositol-3-kinase (PI3K) signaling cascade is essential for cell growth and survival. A series of recent studies indicate that the various PI3K pathway components are targeted by amplification, mutation, and translocation more frequently than any other pathway in cancer patients. The mammalian FOXO subfamily of Forkhead transcription factors is among the best characterized targets of PI3K signaling and consists of three members: FOXO1 (FKHR), FOXO3 (FKHRL1), and FOXO4 (AFX). Growth factor-induced activation of PI3K results in increased downstream activity of the serine⁄threonine kinase AKT, which in turn phosphorylates and inactivates FOXO family of transcription factors as well as other targets to promote cell survival and oppose apoptosis. Under stress conditions that activate FOXOs (e.g. serum-starvation), they translocate from the cytoplasm to the nucleus where they induce or repress transcription of sets of target genes. To interrogate endogenous PI3K⁄AKT signaling, MDA-MB-468 breast cancer cells were engineered with a FOXO responsive element driving beta-lactamase reporter gene expression (DBE-bla). In MDA-MB-468 cells the tumor suppressor PTEN, which normally functions as a negative regulator of PI3K in the absence of growth factor stimulation, is mutationally inactivated resulting in constitutive PI3K⁄AKT signaling. CellSensor® DBE-bla MDA-MB-468 is a clonal population isolated by flow cytometry and tested for assay responsiveness to PI3K inhibitor, PI-103. PI-103 blocks inactivation of downstream FOXO transcription factors by the endogenous constitutive PI3K⁄AKT signaling, thereby allowing for transcriptional upregulation of FOXO-driven beta-lactamase reporter gene expression.
For Research Use Only. Not for use in diagnostic procedures.


System: CellSensor™
Readout: End Point
Reporter: BLA (Beta-Lactamase)
Cell Line: MDA-MB-468
Technique: FRET
Cell State: Dividing Cells
Antagonists: PI-103
Gene Symbol: DBE
Product Size: 1 vial
Primary Agonist: Constitutive
Detection Method: Fluorescent
Druggable Target: Kinases, Signaling Pathway

Contents & storage

CellSensor® DBE-bla MDA-MB-468 cells are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.


Manuals & protocols