PureLink™ Genomic DNA Mini Kit

Description

The PureLink® Genomic DNA Mini Kit enables high-yield, high-purity, genomic DNA (gDNA) extractions from a wide variety of sample types. Use this single kit for genomic DNA purification from blood, tissues, cells, bacteria, swabs, and blood spots, with a familiar silica-based, microcentrifuge spin-column format. Features of the PureLink® Genomic DNA Mini Kit include:

• Kit flexibility—one kit works with a variety of sample types and sizes
• Ultraclean gDNA—minimal contamination of DNA product means successful downstream application
• Improved design—optimized spin-column design and buffer formulation for better yield and purity

Multiple sample sources, one kit
The PureLink® Genomic DNA Mini Kit can be used with many different sample types, each with its own optimized protocol outlined in the manual.

Optimized components and protocols
The PureLink® Genomic DNA Mini Kit includes parts and protocols that improve upon the easy and familiar spin-column methods currently in use (see figure). Columns are newly configured to bind and release DNA more efficiently (see figure). Each sample source uses a specialized lysis procedure and an optimized lysis buffer formulated to enhance proteinase K activity and eliminate protein contamination. A chaotropic salt buffer promotes stable binding of DNA to the column resin, and powerful wash buffers remove all traces of protein and salt. DNA is eluted in a low-salt buffer to allow for pH stabilization of the DNA in storage.

Higher yields and purity
With PureLink® Genomic DNA Kits you can expect high yields of high-purity gDNA (determined by A₂₆₀/A₂₈₀ measurements), no matter the sample type, from bacteria to tissues to blood to cultured cells (see figures). The enhanced PureLink® Lysis and Wash Buffers help to consistently deliver purified gDNA samples with absorbance reading ratios of ~1.9 for both A₂₆₀/A₂₈₀ and A₂₆₀/A₂₃₀, indicating no detectable contamination from carryover of protein or guanidine (see figure).

Figures

Specifications

Sample Type (General):	Bacteria, Blood, Cells, Tissue
Isolation Technology:	Silica Spin Column
Downstream Application:	Genotyping, PCR, Real-Time Quantitative PCR (qPCR), Sequencing, Southern Blotting
High Throughput Compatibility:	Not High Throughput-Compatible (Manual)
Product Line:	PureLink™
Product Size:	50 preps
Shipping Condition:	Room Temperature

Contents & storage