Invitrogen™

PureLink™ Genomic DNA Mini Kit

Catalog number: K182001
Invitrogen™

PureLink™ Genomic DNA Mini Kit

Catalog number: K182001
Catalog Number
K182001
also known as K1820-01
Unit Size
50 preps
Price (USD)
172.00
Your Price:
Availability
***
Quantity
Catalog Number
K182002
also known as K1820-02
Unit Size
250 preps
Price (USD)
728.00
Your Price:
Availability
***
Quantity
Catalog Number
K182000
also known as K1820-00
Unit Size
10 preps
Price (USD)
80.75
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
K182001
also known as K1820-01
50 preps
172.00
Your Price:
***
K182002
also known as K1820-02
250 preps
728.00
Your Price:
***
K182000
also known as K1820-00
10 preps
80.75
Your Price:
***
Product Overview
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Additional Information
Recommendations
The PureLink® Genomic DNA Mini Kit enables high-yield, high-purity, genomic DNA (gDNA) extractions from a wide variety of sample types. Use this single kit for genomic DNA purification from blood, tissues, cells, bacteria, swabs, and blood spots, with a familiar silica-based, microcentrifuge spin-column format. Features of the PureLink® Genomic DNA Mini Kit include:

Kit flexibility—one kit works with a variety of sample types and sizes
Ultraclean gDNA—minimal contamination of DNA product means successful downstream application
Improved design—optimized spin-column design and buffer formulation for better yield and purity

Multiple sample sources, one kit
The PureLink® Genomic DNA Mini Kit can be used with many different sample types, each with its own optimized protocol outlined in the manual.

Optimized components and protocols
The PureLink® Genomic DNA Mini Kit includes parts and protocols that improve upon the easy and familiar spin-column methods currently in use (see figure). Columns are newly configured to bind and release DNA more efficiently (see figure). Each sample source uses a specialized lysis procedure and an optimized lysis buffer formulated to enhance proteinase K activity and eliminate protein contamination. A chaotropic salt buffer promotes stable binding of DNA to the column resin, and powerful wash buffers remove all traces of protein and salt. DNA is eluted in a low-salt buffer to allow for pH stabilization of the DNA in storage.

Higher yields and purity
With PureLink® Genomic DNA Kits you can expect high yields of high-purity gDNA (determined by A260/A280 measurements), no matter the sample type, from bacteria to tissues to blood to cultured cells (see figures). The enhanced PureLink® Lysis and Wash Buffers help to consistently deliver purified gDNA samples with absorbance reading ratios of ∼1.9 for both A260/A280 and A260/A230, indicating no detectable contamination from carryover of protein or guanidine (see figure).
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format
Microcentrifuge Spin Column
Quantity
50 preps
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Prep Scale
100 µl - 1 ml Blood Samples ( Medium-Scale)
Shipping Condition
Room Temperature
Product Line
PureLink™
For Use With (Application)
Genotyping, Real-Time Quantitative PCR (qPCR), Southern Blotting, Sequencing, PCR
Purification Target
Genomic DNA
Sample Type
Bacteria, Tissue, Cells, Blood
No. of Reactions
50 Prep
Isolation Technology
Silica Spin Column
Scale
Mini

Contents & Storage

• 10 ml PureLink™ Genomic Lysis/Binding Buffer
• 9 ml PureLink™ Genomic Digestion Buffer
• 10 ml PureLink™ Genomic Wash Buffer 1
• 7.5 ml PureLink™ Genomic Wash Buffer 2
• 10 ml PureLink™ Genomic Elution Buffer
• 1 ml RNase A (20 mg/ml)
• 1 ml Proteinase K (20 mg/ml)
• 50 each PureLink™ Spin Columns with Collection Tubes
• 100 PureLink™ Collecion Tubes (2.0 ml)

This kit contains enough reagents for 50 DNA preps. Store all components at room temperature. For long-term storage, Proteinase K and RNase A may be stored at 4°C.

Figures

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Certificates

Frequently asked questions (FAQs)

Citations & References