PureLink® Pro 96 Genomic DNA Purification Kit

Catalog number: K182104A

Invitrogen™  Related applications: DNA Extraction

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Description

The PureLink® Pro 96 Genomic DNA Purification Kit enables high-yield, high-purity, genomic DNA (gDNA) extractions from a wide variety of sample types in a high-throughput platform. This single kit allows genomic DNA purification from blood, tissues, cells, bacteria, swabs, and blood spots, using a familiar silica semi-skirted 96-well plate format. Advantages of the PureLink® Pro 96 Genomic DNA Purification Kit:

Kit flexibility—one kit works with a variety of sample types and sizes
Ultraclean gDNA—excellent A260/A280 and A260/A230 absorbance ratios, regardless of sample source
Improved design—optimized, semi-skirted, 96-well plates compatible with centrifugation and most benchtop and robotic vacuum manifolds

Multiple sample sources, one kit
The PureLink® Pro 96 Genomic DNA Purification Kit can be used with many different sample types, each with its own optimized protocol outlined in the manual. Examples of sample sources and amounts that can be used with the PureLink® Pro Genomic DNA Purification Kit include mammalian cells and tissue, FFPE tissue, Oragene™-preserved saliva, mouse and rat tails, non-nucleated whole blood (human, mouse), nucleated whole blood (bird), blood spot on paper, bacterial cells, and yeast cells.

Improved plate design
The PureLink® Pro 96 Genomic DNA Purification Kit provides optimized, semi-skirted, 96-well plates (see figure), for improved performance and results. In addition, the plate nozzles with annular collar prevent cross-contamination of samples and improve drying of the silica membrane to prevent ethanol carryover. Sample processing can be performed manually using a benchtop vacuum manifold, by centrifugation (at ~2 x g), or with an automated liquid handling platform. The semi-skirted plate design is compatible with most vacuum manifolds on robotic workstations.

Higher yields and purity
The PureLink® Pro 96 Genomic DNA Purification Kit outperforms competitor products in both DNA yield and purity for multiple sample types, including blood (see figure). You are also able to get greater yields of higher-purity gDNA (determined by A260/A280 measurements) no matter what your sample type is; from bacteria to tissues to blood to cells (see figures). The enhanced PureLink® Lysis and Wash Buffers help consistently deliver purified gDNA samples with absorbance reading ratios of ~1.9 for both A260/A280 and A260/A230, indicating no contamination carryover of protein or guanidine (see figure).
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Sample Type (General): Bacteria, FFPE & Fixed Samples, Buccal Samples, Cells, Tissue, Blood, Forensic Samples, Yeast
Sample Type (Specific): Cell Cultures, Saliva, Tail Biopsy (Mouse), Bacteria (Gram-Positive), Tail Biopsy (Rat), Buccal Sample, Tissue (Mammalian), Fixed Cells, Whole Blood, Yeast, Insect Cells, Bacteria (Gram-Negative), Mammalian Cells, Tissue (FFPE)
Isolation Technology: Silica Plate
Downstream Application: PCR, Genotyping, Sequencing, Southern Blotting, Next-Generation Sequencing, Real-Time Quantitative PCR (qPCR), Cloning
For Use With (Equipment): Biomek® FX, Robotic Liquid Handling Systems, Centrifuge, TECAN Genesis, Eppendorf epMotion, Vacuum Manifold, TECAN Freedom Evo
High Throughput Compatibility: Not High Throughput-Compatible (Manual), High Throughput-Compatible
Purification Time: 35 min
Format: 96-well plate
Elution Volume - min: 100 µl (vacuum), 50 µl (centrifuge)
Elution Volume - max: 150 µl
System: PureLink™
Quantity: 1 kit
Product Size: 4 x 96 preps
Shipping Condition: Room Temperature

Contents & storage

Contents:
• 80 ml PureLink™ Pro 96 Genomic Lysis/Binding Buffer
• 70 ml PureLink™ Pro 96 Genomic Digestion Buffer
• 2 × 100 ml PureLink™ Pro 96 Genomic Wash Buffer 1
• 2 × 75 ml PureLink™ Pro 96 Genomic Wash Buffer 2
• 160 ml PureLink™ Pro 96 Genomic Elution Buffer
• 8 ml RNase A (20 mg/ml)
• 8 ml PureLink™ Pro 96 Proteinase K (20 mg/ml)
• 4 PureLink™ Genomic DNA Filter Plate
• 2 × 6 96 Deep Well Blocks
• 20/pack PureLink™ Foil Tape

Store all components at room temperature. For long-term storage, Proteinase K and RNase A may be stored at 4°C.

Documents

Manuals & protocols

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