PureLink™ Pro 96 Genomic DNA Purification Kit

Description

The PureLink® Pro 96 Genomic DNA Purification Kit enables high-yield, high-purity, genomic DNA (gDNA) extractions from a wide variety of sample types in a high-throughput platform. This single kit allows genomic DNA purification from blood, tissues, cells, bacteria, swabs, and blood spots, using a familiar silica semi-skirted 96-well plate format. Advantages of the PureLink® Pro 96 Genomic DNA Purification Kit:

• Kit flexibility—one kit works with a variety of sample types and sizes
• Ultraclean gDNA—excellent A₂₆₀/A₂₈₀ and A₂₆₀/A₂₃₀ absorbance ratios, regardless of sample source
• Improved design—optimized, semi-skirted, 96-well plates compatible with centrifugation and most benchtop and robotic vacuum manifolds

Multiple sample sources, one kit
The PureLink® Pro 96 Genomic DNA Purification Kit can be used with many different sample types, each with its own optimized protocol outlined in the manual. Examples of sample sources and amounts that can be used with the PureLink® Pro Genomic DNA Purification Kit include mammalian cells and tissue, FFPE tissue, Oragene™-preserved saliva, mouse and rat tails, non-nucleated whole blood (human, mouse), nucleated whole blood (bird), blood spot on paper, bacterial cells, and yeast cells.

Improved plate design
The PureLink® Pro 96 Genomic DNA Purification Kit provides optimized, semi-skirted, 96-well plates (see figure), for improved performance and results. In addition, the plate nozzles with annular collar prevent cross-contamination of samples and improve drying of the silica membrane to prevent ethanol carryover. Sample processing can be performed manually using a benchtop vacuum manifold, by centrifugation (at ~2 x g), or with an automated liquid handling platform. The semi-skirted plate design is compatible with most vacuum manifolds on robotic workstations.

Higher yields and purity
The PureLink® Pro 96 Genomic DNA Purification Kit outperforms competitor products in both DNA yield and purity for multiple sample types, including blood (see figure). You are also able to get greater yields of higher-purity gDNA (determined by A₂₆₀/A₂₈₀ measurements) no matter what your sample type is; from bacteria to tissues to blood to cells (see figures). The enhanced PureLink® Lysis and Wash Buffers help consistently deliver purified gDNA samples with absorbance reading ratios of ~1.9 for both A₂₆₀/A₂₈₀ and A₂₆₀/A₂₃₀, indicating no contamination carryover of protein or guanidine (see figure).

Figures

Spécifications

Sample Type (General):	Bacteria, Blood, Buccal Samples, Cells, FFPE & Fixed Samples, Forensic Samples, Tissue, Yeast
Sample Type (Specific):	Bacteria (Gram-Negative), Bacteria (Gram-Positive), Buccal Sample, Cell Cultures, Fixed Cells, Insect Cells, Mammalian Cells, Saliva, Tail Biopsy (Mouse), Tail Biopsy (Rat), Tissue (FFPE), Tissue (Mammalian), Whole Blood, Yeast
Isolation Technology:	Silica Plate
Downstream Application:	Cloning, Genotyping, Next-Generation Sequencing, PCR, Real-Time Quantitative PCR (qPCR), Sequencing, Southern Blotting
For Use With (Equipment):	Biomek® FX, Centrifuge, Eppendorf epMotion, Robotic Liquid Handling Systems, TECAN Freedom Evo, TECAN Genesis, Vacuum Manifold
High Throughput Compatibility:	High Throughput-Compatible, Not High Throughput-Compatible (Manual)
Purification Time:	35 min
Format:	96-well plate
Elution Volume - min:	100 µl (vacuum), 50 µl (centrifuge)
Elution Volume - max:	150 µl
Product Line:	PureLink™
Product Size:	4 x 96 preps
Quantity:	1 kit
Shipping Condition:	Room Temperature
System:	PureLink™

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