The Tango™ F2RL1-bla U2OS cells contain the human Coagulation factor II (thrombin) receptor-like 1 (F2RL1) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin⁄TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.
The Tango™ F2RL1-bla U2OS cells are functionally validated for Z' and EC50 concentrations of SLIGRL. In addition, Tango™ F2RL1-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.
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For Research Use Only. Not for use in diagnostic procedures.
Specifications
Accession Number
NM_005242.3
Assay Entry
Cell-based beta-arrestin recruitment
Cell State
Dividing Cells
Cytoplasmic Tail
Tail from AVPR2 GPCR
Detection Method
Fluorescent
For Use With (Application)
FRET
Gene Symbol
F2RL1
Human Protein Class
G-Protein Coupled Receptor (GPCR)
Population Status
Single Cell Clone Origin
Product Line
GeneBLAzer™, Tango™
Readout
End Point
Target Entry
F2RL1
Cell Line
U2OS
System Type
Tango™
Druggable Target
GPCRs
Contents & Storage
Contents: K1830 Tango™ F2RL1-bla U2OS cells (LiveBLAzer™-FRET B⁄G Loading Kit, Solution D and other materials are required separately; please refer to the protocol). Includes: • Tango™ F2RL1-bla U2OS cells • Tango™ F2RL1-bla U2OS Assay Protocol • Certificate of Analysis
Storage: The Tango™ F2RL1-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.