Thermo Scientific™

LanthaScreen™ IGF-1R GripTite™ Cell Line

Catalog number: K1834
Thermo Scientific™

LanthaScreen™ IGF-1R GripTite™ Cell Line

Catalog number: K1834
Catalog Number
K1834
Unit Size
1 vial
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
K18341 vial
-
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The IGF-1R⁄PI3K⁄AKT pathway mediates signals for cell growth, cell survival, transcription, translation, and glucose uptake. IGF-1R is a receptor tyrosine kinase (RTK) that resides at the top of the pathway. Upon growth factor binding extracellularly, this RTK undergoes auto-phosphorylation at multiple intracellular tyrosine residues (resulting in pathway activation). The LanthaScreen® IGF-1R GripTite™ (HEK 293 MSR) cellular assay utilizes a human cell line that constitutively expresses IGF-1R fusion proteins with yellow fluorescent protein (YFP). This kinase target was introduced using lipid transfection and these cells are a clonal population isolated by FACS, using YFP fluorescence as a sorting marker and Blasticidin to maintain cells under selection. Using this cell line, a homogenous immunoassay was developed with a time-resolved FRET (TR-FRET) readout in which the insulin-induced phosphorylation of multiple tyrosine residues on IGF-1R is detected in cell lysates using a generic terbium-labeled phosphospecific antibody (Tb-anti-pY20). This cell line has been validated with different stimuli⁄inhibitors and shows correct EC50 ⁄ IC50 values. Moreover, this assay has been optimized for performance under variable experimental conditions (including cell plating density, agonist stimulation time, DMSO tolerance and assay development time) and displays excellent statistical data (Z' > 0.6) and good signal-to-background.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Antagonists
IGF-1R Inhibitor II
Assay Entry
Cell-based terbium phospho-specific activity (stable)
Cell State
Dividing Cells
Detection Method
Fluorescent
For Use With (Application)
TR-FRET
Gene Symbol
IFG-1R
Human Protein Class
Membrane Proteins
Primary Agonist
IGF-1
Product Line
LanthaScreen™
Readout
End Point
Reporter Gene
GFP (EmGFP)
Target Entry
IGF-1R
Type
Cell Line
Cell Line
293 GripTite MSR
System Type
LanthaScreen™
Druggable Target
Kinases

Contents & Storage

LanthaScreen™ IGF-1R GripTite™ cells are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.

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