Tango™ GPR1-bla U2OS cells contain the human G protein-coupled receptor 1 (GPR1) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin⁄TEV protease fusion protein and the beta-lactamase reporter gene under the control of a UAS response element. The Tango™ GPR1-bla U2OS cells have been functionally validated for Z’ factor and EC50 concentrations of Chemerin (Figure 1). In addition, Tango™ GPR1-bla U2OS cells have been tested for assay performance under variable conditions.
For Research Use Only. Not for use in diagnostic procedures.
Cell-based beta-arrestin recruitment
For Use With (Application)
Human Protein Class
G-Protein Coupled Receptor (GPCR)
Single Cell Clone Origin
Contents & Storage
K1839 Tango™ GPR1-UAS-bla CHO-K1 cells (LiveBLAzer™-FRET B⁄G Loading Kit, Solution D and other materials are required separately; please refer to the protocol). Includes: • Tango™ GPR1-UAS-bla CHO-K1 cells • Tango™ GPR1-UAS-bla CHO-K1 Assay Protocol • Certificate of Analysis The Tango™ GPR1-UAS-bla CHO-K1 cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.