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Thermo Scientific™

Tango™ GPR23-bla U2OS Cells

Catalog number:  K1841

Related applications:

Pharma & Biopharma

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Description

Tango™ GPR23-bla U2OS cells contain the human G protein-coupled receptor 23 (GPR23) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin⁄TEV protease fusion protein and the beta-lactamase reporter gene under the control of a UAS response element. The Tango™ GPR23-bla U2OS cells have been functionally validated for Z’ factor and a functional response to FBS (Figure 2). In addition, Tango™ GPR23-bla U2OS cells have been tested for assay performance under variable conditions.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Accession Number: NM_005296.1
Assay Entry: Cell-based beta-arrestin recruitment
Cell Line: U2OS
Cell State: Dividing Cells
Cytoplasmic Tail: Native Tail
Detection Method: Fluorescent
Druggable Target: GPCRs
Gene Symbol: GPR23
Human Protein Class: G-Protein Coupled Receptor (GPCR)
Population Status: Single Cell Clone Origin
Product Line: Tango™
Readout: End Point
System Type: Tango™
Target Entry: GPR23
Technique: FRET

Contents & storage

K1841 Tango™ GPR23-UAS-bla CHO-K1 cells
(LiveBLAzer™-FRET B⁄G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
Includes:
• Tango™ GPR23-UAS-bla CHO-K1 cells
• Tango™ GPR23-UAS-bla CHO-K1 Assay Protocol
• Certificate of Analysis
The Tango™ GPR23-UAS-bla CHO-K1 cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.