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Thermo Scientific™

Tango™ GPR120S-bla U2OS Cells

Catalog number:  K1845

Related applications:

Pharma & Biopharma

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Description

Tango™ GPR120 (short)-bla U2OS cells contain the human GPR120 (short) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin⁄TEV protease fusion protein and the beta-lactamase reporter gene under the control of a UAS response element. The Tango™ GPR120 (short)-bla U2OS cells have been functionally validated for Z’ factor and EC50 concentrations of GW9508 (Figure 1). In addition, Tango™ GPR120 (short)-bla U2OS cells have been tested for assay performance under variable conditions.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Accession Number: NM_181745.2
Assay Entry: Cell-based beta-arrestin recruitment
Cell Line: U2OS
Cell State: Dividing Cells
Cytoplasmic Tail: Native Tail
Detection Method: Fluorescent
Druggable Target: GPCRs
Gene Symbol: GPR120S
Human Protein Class: G-Protein Coupled Receptor (GPCR)
Population Status: Single Cell Clone Origin
Product Line: Tango™
Readout: End Point
System Type: Tango™
Target Entry: GPR120S
Technique: FRET

Contents & storage

Note: LiveBLAzer™-FRET B/G Loading Kit, Solution D, and other materials are required separately—please refer to the protocol.

Contents:
• Tango™ GPR120S-bla U2OS cells
• Tango™ GPR120S-bla U2OS cell Assay Protocol
• Certificate of Analysis

The Tango™ GPR120S-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.