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Thermo Scientific™

GeneBLAzer™ HTR2B-NFAT-bla CHO-K1 Cells

Catalog number:  K1847

Related applications:

Pharma & Biopharma

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Description

GeneBLAzer® HTR2B-NFAT-bla CHO-K1 cells contain the human 5-hydroxytryptamine (serotonin) receptor 2B (HTR2B), (Accession # NM_000867) stably integrated into the CellSensor® NFAT-bla CHO-K1 cell line. CellSensor® NFAT-bla CHO-K1 cells (Cat. no.) contain a beta-lactamase reporter gene under control of the NFAT Response Element. The GeneBLAzer® HTR2B-NFAT-bla CHO-K1 cells are functionally validated for Z’-factor and EC50 concentrations of 5-HT (Figure 1). In addition, GeneBLAzer® HTR2B-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Accession Number: NM_000867.2
Assay Entry: Cell-based beta lactamase reporter gene
Cell Line: CHO-K1
Cell State: Dividing Cells
Detection Method: Fluorescent
Druggable Target: GPCRs
Gene Symbol: HTR2B
Human Protein Class: G-Protein Coupled Receptor (GPCR)
Population Status: Single Cell Clone Origin
Product Line: GeneBLAzer®
Readout: End Point
System Type: GeneBLAzer™
Target Entry: HTR2B
Technique: FRET

Contents & storage

K1847 GeneBLAzer® HTR2B-NFAT-bla CHO-K1 cells
(LiveBLAzer™-FRET B⁄G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
Includes:
• GeneBLAzer® HTR2B-NFAT-bla CHO-K1 cells
• GeneBLAzer® HTR2B-NFAT-bla CHO-K1 Assay Protocol
• Certificate of Analysis
The GeneBLAzer® HTR2B-NFAT-bla CHO-K1 cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.