Tango™ GPR35-bla U2OS cells contain the human G-protein coupled receptor 35 (GPR35) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin⁄TEV protease fusion protein and the beta-lactamase reporter gene under the control of a UAS response element. The Tango™ GPR35-bla U2OS cells have been functionally validated for Z’ factor and EC50 concentrations of Zaprinast. In addition, Tango™ GPR35-bla U2OS cells have been tested for assay performance under variable conditions.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Accession Number
NM_005301.2
Assay Entry
Cell-based beta-arrestin recruitment
Cell State
Dividing Cells
Cytoplasmic Tail
Native Tail
Detection Method
Fluorescent
For Use With (Application)
FRET
Human Protein Class
G-Protein Coupled Receptor (GPCR)
Population Status
Single Cell Clone Origin
Product Line
Tango™
Readout
End Point
Target Entry
GPR35
Cell Line
U2OS
System Type
Tango™
Druggable Target
GPCRs
Contents & Storage
Tango™ GPR35-bla U2OS cells cells are shipped on dry ice, 8 x 10^6 cells⁄vial. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.