The S.N.A.P.™ MidiPrep Kit is a medium-scale plasmid DNA purification kit designed for fast and easy purification of pure plasmid DNA from 10-100 ml of overnight culture (Figure 1). The S.N.A.P.™ MidiPrep procedure takes just 40 minutes. You simply:
• Lyse cells and filter debris with the S.N.A.P.™ filtering column • Apply flow-through to the S.N.A.P.™ DNA binding column • Wash the column • Elute pure, supercoiled plasmid DNA in water or TE buffer
Plasmid DNA purified with the S.N.A.P.™ MidiPrep Kit is ready to use right off the column. No precipitation is necessary. Plasmid is eluted in TE or water at ∼200 ng/μl-perfect for subsequent applications including mammalian cell transfection. Typical yields are shown in Table 1.
For Research Use Only. Not for use in diagnostic procedures.
Final Product Type
For Use With (Application)
Sequencing, Cloning, Transformation, Transfection, In Vitro Transcription
Not High-throughput Compatible (Manual)
No. of Reactions
100-200 μg (Medium-Scale) Plasmid DNA
Plasmid DNA MidiPrep Kit
Contents & Storage
Store the S.N.A.P.™ MidiPrep filtering columns, S.N.A.P.™ MidiPrep DNA binding columns, lysis buffer, binding buffer, wash buffer, and 4X final wash at room temperature. Store the resuspension buffer, precipitation salt, and RNase A at +4°C. All reagents are guaranteed stable for 6 months when properly stored.