Invitrogen™

PureLink™ HQ Mini Plasmid DNA Purification Kit

Catalog number:  K210001

 Related applications:

DNA Extraction

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Description

The PureLink HQ Mini Plasmid Purification Kit is designed for the isolation of high-quality plasmid DNA that is suitable for restriction enzyme digestion, PCR, sequencing, bacterial cell transformation, and mammalian cell transfection. Using the kit, plasmid DNA can be isolated from varying amounts of bacterial cells.

The PureLink HQ Mini Plasmid Purification Kit has the following advantages:
• Higher spin column capacity for plasmid DNA as compared to other commercially available plasmid purification systems
• Designed to isolate high-quality plasmid DNA in less than an hour
• Minimal genomic DNA contamination of the purified sample
• Reliable performance of the purified DNA in downstream applications

Process overview
Bacterial cells are pelleted, resuspended, and lysed. The lysate is then neutralized and conditions are adjusted for subsequent binding. After clarification by centrifugation, the lysate is processed through the PureLink spin column. The DNA binds to the silica-based membrane in the column, and impurities are removed by a single wash step. The DNA is then eluted in Elution Buffer or water.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Sample Type (General): Bacteria
Downstream Application: Cloning, PCR, Sequencing, Transfection, Transformation
Final Product: Plasmid DNA
Isolation Technology: Silica Spin Column
Product Line: PureLink™
Product Size: 100 preps
Sample Type (Specific): Bacterial Culture
Shipping Condition: Room Temperature

Contents & storage

The PureLink HQ Mini Plasmid DNA Purification Kit includes sufficient PureLink columns, collection tubes, elution tubes, and reagents for 100 purifications. Store components at room temperature. Store Resuspension Buffer after addition of RNase A at +4°C. All reagents are guaranteed stable for 6 months when properly stored.

Documents

Manuals & protocols