Invitrogen™

PureLink™ HiPure Plasmid Miniprep Kit

Catalog number:  K210003

Related applications:

DNA Extraction

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Description

The PureLink® HiPure Plasmid Miniprep Kit allows isolation of high yields of highly pure plasmid DNA from E.coli in less than 2 hours, without the use of any organic solvents or cesium chloride, and contains low endotoxin levels.
• Fast – Highly-quality purified plasmid DNA in under 2 hours
• Efficient – Increased yields with low endotoxin levels

Plasmid DNA of High Purity
The PureLink® HiPure Plasmid Miniprep Kit is designed to efficiently isolate plasmid DNA from E.coli in 1.5-2 hours. The kit employs anion-exchange resin composed of small particles with a uniform pore size to purify plasmid DNA to a level equivalent to two passes through CsCl gradients (Figure 1). The need for additional steps to remove contaminants such as phenol, chloroform, and CsCl are eliminated, minimizing exposure to and disposal of hazardous materials.

High Yield and Efficient Endotoxin Removal
PureLink® HiPure Plasmid Miniprep Kit allows purification of up to 30 µg of high quality plasmid DNA from 1-3 ml overnight E.coli cultures in approximately 1 hour, with an endotoxin level of 0.1-1 EU/ µg, when cloning high copy number plasmids (Table 1). Typically, DNA isolated using the PureLink® HiPure Plasmid Miniprep Kit has an A260/A280 > 1.80 when samples are diluted in Tris-HCl (pH 7.5) indicating that the DNA is reasonably free of proteins that could interfere with downstream applications.

Isolated DNA can be used for a Number of Downstream Applications:
The purified DNA is ultrapure and suitable for downstream applications, including those requiring the highest purity DNA, such as mammalian transfections (Figure 2), automated and manual DNA sequencing, PCR amplification, in vitro transcription, bacterial cell transformation, cloning, and labeling.

For Research Use Only. Not intended for diagnostic procedures.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Final Product Type: BAC DNA, Plasmid DNA
For Use With (Application): Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
Isolation Technology: Silica Spin Column
Purification Target: BAC DNA, Plasmid DNA
Sample Type: Bacterial Culture
Includes:
  • 50mL Resuspension Buffer (R3)
  • 550 μl RNase A
  • 50mL Lysis Buffer (L7)
  • 40mL Precipitation Buffer (N3)
  • 250mL Equilibration Buffer (EQ1)
  • 500mL Wash Buffer (W8)
  • 90mL Elution Buffer (E4)
  • 15mL TE Buffer
  • 100 HiPure Columns
Quantity: 100 preps
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Prep Scale: < 100 µg (Small-Scale) Plasmid DNA
Shipping Condition: Room Temperature
Product Line: PureLink™

Contents & storage

• 50 ml Resuspension Buffer (R3)
• 550 µl RNase A
• 50 ml Lysis Buffer (L7)
• 40 ml Precipitation Buffer (N3)
• 250 ml Equilibration Buffer (EQ1)
• 500 ml Wash Buffer (W8)
• 90 ml Elution Buffer (E4)
• 15 ml TE Buffer
• 100 HiPure Columns

Store all components at room temperature.