The a disintegrin and metalloproteinase (ADAM)-17 releases pro-inflammatory cytokines, including tumor necrosis factor-α, and several epidermal growth factor receptor ligands from cells. ADAM17 is post-translationally regulated by the inactive rhomboid pseudoproteases iRhom1 and iRhom2, and dysregulation of this signaling axis contributes to diseases ranging from chronic inflammation to cancer. Here, ... More
Tools for genetic engineering and gene expression control in Novosphingobium aromaticivorans and Rhodobacter sphaeroides.
AuthorsHall AN,Hall BW,Kinney KJ,Olsen GG,Banta AB,Noguera DR,Donohue TJ,Peters JM
JournalApplied and environmental microbiology
PubMed ID39324814
Alphaproteobacteria have a variety of cellular and metabolic features that provide important insights into biological systems and enable biotechnologies. For example, some species are capable of converting plant biomass into valuable biofuels and bioproducts that have the potential to contribute to the sustainable bioeconomy. Among the Alphaproteobacteria, Novosphingobium aromaticivorans, Rhodobacter ... More
Protocol to evaluate the inflammatory response in human macrophages induced by SARS-CoV-2 spike-pseudotyped VLPs.
AuthorsOuyang MJ,Ao Z,Olukitibi TA,Yao XJ
JournalSTAR protocols
PubMed ID36853685
The excessive release of pro-inflammatory cytokines in COVID-19 patients is deleterious to organs. The contribution of SARS-CoV-2 spike protein (S) to the inflammatory response is essential to understand its pathogenesis and virulence. Here, we present a protocol to produce and characterize HIV- and SARS-CoV-2-based virus-like particles and then evaluate the inflammatory ... More
CRISPR/Cas9-mediated tissue-specific knockout and cDNA rescue using sgRNAs that target exon-intron junctions in Drosophila melanogaster.
AuthorsChilian M,Vargas Parra K,Sandoval A,Ramirez J,Yoon WH
JournalSTAR protocols
PubMed ID35719725
In this protocol, we take CRISPR/Cas9 and Gal4/UAS approaches to achieve tissue-specific knockout in parallel with rescue of the knockout by cDNA expression in Drosophila. We demonstrate that guide RNAs targeting the exon-intron junction of target genes cleave the genomic locus of the genes, but not UAS-cDNA transgenes, in a ... More
The inhibitory receptor TIM-3 limits activation of the cGAS-STING pathway in intra-tumoral dendritic cells by suppressing extracellular DNA uptake.
Blockade of the inhibitory receptor TIM-3 shows efficacy in cancer immunotherapy clinical trials. TIM-3 inhibits production of the chemokine CXCL9 by XCR1( ) classical dendritic cells (cDC1), thereby limiting antitumor immunity in mammary carcinomas. We found that increased CXCL9 expression by splenic cDC1s upon TIM-3 blockade required type I interferons ... More
Multicopy blaOXA-58 gene as a source of high-level resistance to carbapenems in Acinetobacter baumannii.
AuthorsBertini A, Poirel L, Bernabeu S, Fortini D, Villa L, Nordmann P, Carattoli A,
JournalAntimicrob Agents Chemother
PubMed ID17438042
'The mechanisms at the origin of heterogeneous carbapenem resistance levels observed among Acinetobacter baumannii isolates collected in 2005 in a large University Hospital of Rome, Italy, were investigated. These isolates were related and possessed similar plasmids carrying the carbapenem-hydrolyzing oxacillinase gene bla(OXA-58) but showed variable levels of resistance to carbapenems. ... More
Role of LRAT on the retinoid isomerase activity and membrane association of Rpe65.
AuthorsJin M, Yuan Q, Li S, Travis GH,
JournalJ Biol Chem
PubMed ID17504753
Absorption of a photon by a vertebrate opsin pigment induces 11-cis to all-trans isomerization of its retinaldehyde chromophore. Restoration of light sensitivity to the bleached opsin requires chemical re-isomerization of the chromophore via an enzyme pathway called the visual cycle. The retinoid isomerase in this pathway is Rpe65, a membrane-associated ... More