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Invitrogen™

PureLink™ HiPure Expi Plasmid Megaprep Kit

Catalog number:  K210008XP

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Description

The PureLink™ HiPure Expi Plasmid Megaprep Kit enables isolation of the highest levels of transfection-grade plasmid DNA from a single E.coli mega prep using an enhanced anion exchange resin. The PureLink™ HiPure Plasmid Megaprep Kit also includes vacuum filtration columns that provide bacterial filtration without centrifugation, enabling extremely fast process times. The PureLink™ HiPure Expi Plasmid Megaprep Kit typically isolates 4 mg of high quality, ultrapure plasmid DNA with inherently low endotoxin levels from 500 mL of bacterial culture.

Advantages of the PureLink™ HiPure Expi Plasmid Megaprep Purification Kit include:
• High yield—isolate over 5 mg of high quality plasmid DNA from a single purification using 1 L of bacterial culture volume
• Purity—inherently low endotoxin levels (typically 0.1–1.0 EU/µg) and A260/280 >1.8, making it ideal for mammalian cell transfection
• Simple and super-fast protocol—with vacuum assisted protocol, typical plasmid isolation takes as little as 90 minutes

HiPure Expi anion exchange chromatography for plasmid purification
PureLink™ HiPure Expi plasmid purification kits use a novel, proprietary anion-exchange resin to enable the purification of the highest levels of transfection-grade plasmid DNA. The enhanced resin combines large capacity with fast flow rate, high resolution, and efficient endotoxin removal. Plasmid preparations are typically completed in less than 2 hours with purity equal to two CsCl gradients. PureLink™ HiPure Expi plasmid purification kits enable the quickest process for obtaining the highest amount of transfection-grade plasmid DNA.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Application): Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
Purification Target: Plasmid DNA
Isolation Technology: Anion Exchange Resin, Filter, Vacuum
High-throughput Compatibility: Manual Protocols
Prep Scale: 4 mg plasmid DNA
Shipping Condition: Approved for shipment at Room Temperature or on Wet Ice
Product Line: PureLink™

Contents & storage

• Equilibration Buffer
• Resuspension Buffer
• Lysis Buffer
• Precipitation Buffer
• RNase A
• Wash Buffer
• Elution Buffer
• TE Buffer
• Expi HiPure Mega Filtration Column
• Expi HiPure Mega DNA Binding Column

Store all components at room temperature upon receipt.