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Invitrogen™

PureLink™ HiPure Expi Plasmid Gigaprep Kit

Catalog number: K210009XP
Invitrogen™

PureLink™ HiPure Expi Plasmid Gigaprep Kit

Catalog number: K210009XP
Catalog Number
K210009XP
Unit Size
2 preps
Price (USD)
Price: 264.00
Your Price:
Availability
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Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
K210009XP2 preps
Price: 264.00
Your Price:
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The PureLink™ HiPure Expi Plasmid Gigaprep Kit enables isolation of the highest levels of transfection-grade plasmid DNA from a single E.coli giga prep using an enhanced anion exchange resin. The PureLink™ HiPure Plasmid Gigaprep Kit also includes vacuum filtration columns that provide bacterial filtration without centrifugation, enabling extremely fast process times. The PureLink™ HiPure Expi Plasmid Gigaprep Kit typically isolates 12 mg of high quality, ultrapure plasmid DNA with inherently low endotoxin levels from 2.5 L of bacterial culture.

Advantages of the PureLink™ HiPure Expi Plasmid Gigaprep Kit include:
• High yield—isolate up to 14 mg of high quality plasmid DNA from a single purification using 5 L of bacterial culture volume
• Purity—inherently low endotoxin levels (typically 0.1–1.0 EU/µg) and A260/280 >1.8, making it ideal for mammalian cell transfection
• Simple and super-fast protocol—with vacuum assisted protocol, typical plasmid isolation in as little as 90 minutes

HiPure Expi anion exchange chromatography for plasmid purification
PureLink™ HiPure Expi plasmid purification kits use a novel, proprietary anion-exchange resin to enable the purification of the highest levels of transfection-grade plasmid DNA. The enhanced resin combines large capacity with fast flow rate, high resolution, and efficient endotoxin removal. Plasmid preparations are typically completed in less than 2 hours with purity equal to two CsCl gradients. PureLink™ HiPure Expi plasmid purification kits enable the quickest process for obtaining the highest amount of transfection-grade plasmid DNA.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format
Kit
Isolation Technology
Anion Exchange Resin, Filter, Vacuum
Sample Type
Bacterial Culture
For Use With (Application)
Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
High-throughput Compatibility
Manual Protocols
No. of Reactions
2 Preps
Prep Scale
12 mg plasmid DNA
Product Line
PureLink™
Product Type
Plasmid GigaPrep Kit
Quantity
2 Preps
Shipping Condition
Approved for shipment at Room Temperature or on Wet Ice
Target
Plasmid DNA
Test Time
2 hr.

Contents & Storage

• Equilibration Buffer
• Resuspension Buffer
• Lysis Buffer
• Precipitation Buffer
• RNase A
• Wash Buffer
• Elution Buffer
• TE Buffer
• Expi HiPure Giga Filtration Column
• Expi HiPure Giga DNA Binding Column

Store all components at room temperature upon receipt.

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