Invitrogen™

PureLink™ Quick Plasmid Miniprep Kit

Catalog number: K210010
Invitrogen™

PureLink™ Quick Plasmid Miniprep Kit

Catalog number: K210010
Catalog Number
K210010
also known as K2100-10
Unit Size
50 preps
Price (USD)
84.25
Your Price:
Availability
***
Quantity
Catalog Number
K210011
also known as K2100-11
Unit Size
250 preps
Price (USD)
370.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
K210010
also known as K2100-10
50 preps
84.25
Your Price:
***
K210011
also known as K2100-11
250 preps
370.00
Your Price:
***
Product Overview
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Additional Information
Recommendations
The PureLink® Quick Plasmid Miniprep Kit is designed for rapid isolation of sequencing-grade plasmid DNA from E.coli in 30-45 minutes, using silica membrane columns. Features of the PureLink® Quick Plasmid Miniprep Kit:

Fast—ready-to-use plasmid DNA typically in 30-45 minutes
Flexible—formats work with vacuum and centrifugation platforms

Reliable and cost-effective isolation of plasmid DNA
The PureLink® Quick Plasmid Miniprep Kit allows isolation of high-quality plasmid DNA from 1–5 mL cultures in 30-45 minutes, using the PureLink® spin columns. These columns use a unique silica membrane to achieve high yields of up to 40 µg of sequencing-grade plasmid DNA. Cells are lysed using an alkaline/SDS procedure and the lysate is applied to a silica membrane column that selectively binds plasmid DNA. Contaminants are removed with Wash Buffer, and the plasmid DNA is eluted in TE Buffer. The eluted DNA is suitable for all routine downstream applications.

Compatible with flexible formats
The PureLink® Quick Plasmid Miniprep Kit can be used with a centrifuge or a vacuum manifold for purifying plasmid DNA. Typically, DNA isolated using the PureLink® Quick Plasmid Miniprep Kit has an A260/A280 of >1.80 when samples are diluted in Tris-HCl (pH 7.5), indicating that the DNA is reasonably free of proteins that could interfere with downstream applications.

Isolated DNA can be used for a variety of downstream applications
Plasmid DNA purified using PureLink® Quick Plasmid Miniprep Kit is suitable for all routine downstream applications, including bacterial and mammalian cell transformations, DNA sequencing, restriction enzyme digestion, cloning, and PCR.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Concentration
20 mg/mL
Format
Spin Column
Quantity
50 preps
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Prep Scale
< 100 µg (Small-Scale) Plasmid DNA
Shipping Condition
Room Temperature
Product Line
PureLink™
For Use With (Application)
Next-Generation Sequencing, Cloning, Sequencing, Nucleic Acid Labeling, PCR, Transformation
Final Product Type
Plasmid DNA
Purification Target
Plasmid DNA
Sample Type
Bacterial Culture
Purification Time
30 min to 45 min
No. of Reactions
50 Prep
Isolation Technology
Silica Spin Column

Contents & Storage

• Resuspension buffer
• RNase A (20 mg/mL)
• Lysis buffer
• Precipitation buffer
• Wash buffers
• TE buffer
• Spin columns in wash tubes
• Elution tubes

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Frequently asked questions (FAQs)

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