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Invitrogen™

PureLink™ HiPure Plasmid Filter Midiprep Kit

Catalog number: K210014
Invitrogen™

PureLink™ HiPure Plasmid Filter Midiprep Kit

Catalog number: K210014
Catalog Number
K210014
also known as K2100-14
Unit Size
25 preps
Price (USD)
Price: 377.00
Online price: 332.65
Your price:
Availability
-
Quantity
Catalog Number
K210015
also known as K2100-15
Unit Size
50 preps
Price (USD)
Price: 744.00
Online price: 655.65
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
K210014
also known as K2100-14
25 preps
Price: 377.00
Online price: 332.65
Your price:
-
K210015
also known as K2100-15
50 preps
Price: 744.00
Online price: 655.65
Your price:
-
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The PureLink™ HiPure Plasmid Filter Midiprep Kit is designed to isolate plasmid DNA of the highest purity from 25 to 100 mL of an overnight bacterial culture. The PureLink™ HiPure Plasmid Filter Kits include HiPure Filter Columns, which combine filtration for clarification of the bacterial lysate with anion-exchange resin purification of plasmid DNA, in a single unit. The resulting plasmid DNA is of a quality equivalent to that obtained by purification with two passes through cesium chloride gradients—the most rigorous method for DNA purification. Purify high-quality DNA in less than two hours for transfections and other applications with no need for additional steps to remove contaminants like RNA, proteins, or endotoxins. Additionally, the elimination of phenol, chloroform, ethidium bromide, and cesium chloride from the protocol minimizes exposure to, and disposal of hazardous materials. The PureLink™ HiPure Plasmid Filter Kits provide:

Simple protocols—bacterial lysate clarification without centrifugation
Fast results—save time by purifying plasmid DNA in less than two hours
High yields—up to 200 μg for midipreps and up to 850 μg for maxipreps (see table)
Reliable performance—the high-quality plasmid DNA purified with these kits is suitable for a wide variety of applications

How it works
The addition of a HiPure Filter Column to the PureLink™ HiPure Plasmid Kits provides rapid clearing of the bacterial lysate without the need for centrifugation (see figure). The lysate filtration cartridge is integrated into the DNA-binding column for a combined step of lysate clarification and plasmid DNA binding directly to the anion-exchange resin. A single column wash removes impurities such as RNA, proteins, metabolites, and other low molecular weight molecules; the ultrapure plasmid DNA is then eluted with a high-salt buffer. The DNA is then desalted and concentrated with an isopropanol precipitation step, then collected by centrifugation. The entire protocol can be completed in about two hours.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Column Type
Filter Column
Format
Column
Isolation Technology
Anion Exchange Resin
Sample Type
Bacterial Culture
Elution Volume
5 mL
Final Product Type
Plasmid DNA
For Use With (Application)
Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
High-throughput Compatibility
Not High-throughput Compatible (Manual)
No. of Reactions
25 Preps
Plasmid
<40kb, Low Copy Plasmid, High Copy Plasmid, BAC
Prep Scale
100-200 μg (Medium-Scale) Plasmid DNA
Product Line
PureLink™
Product Type
Plasmid Filter MidiPrep Kit
Quantity
25 Preps
Purity
Transfection Grade
Shipping Condition
Room Temperature
Target
Plasmid DNA
System Type
PureLink™
Test Time
90 min.
Yield
350 μg

Contents & Storage

The PureLink™ HiPure Plasmid Filter Kits include Equilibration Buffer (EQ1), Resuspension Buffer (R3), Lysis Buffer (L7), Precipitation Buffer (N3), RNase A, Wash Buffer (W8), Elution Buffer (E4), TE Buffer (TE), and HiPure Filter Columns. Store all components at room temperature upon receipt.

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