Invitrogen™

PureLink™ HiPure BAC Buffer Kit

Catalog number: K210018
Invitrogen™

PureLink™ HiPure BAC Buffer Kit

Catalog number: K210018
Catalog Number
K210018
also known as K2100-18
Unit Size
1 kit
Price (USD)
Price: 139.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
K210018
also known as K2100-18
1 kit
Price: 139.00
Your Price:
***
Product Overview
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Additional Information
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Recommendations
The PureLink™ HiPure BAC Buffer Kit provides additional buffers and reagents for performing BAC (Bacterial Artificial Chromosome) DNA isolation using the PureLink™ HiPure Plasmid Kits. The PureLink™ HiPure Plasmid Kits utilize a proprietary anion-exchange resin which allows you to purify high-quality BAC DNA from E. coli cultures. BAC DNA requires the use of large bacterial culture volumes to obtain a reasonable DNA yield. The additional buffers and reagents needed for working with larger bacterial cultures are provided in this Buffer Kit. From a 100 ml culture, you can expect to purify ∼40 µg of BAC DNA. The PureLink™ HiPure BAC Buffer Kit provides reagents sufficient for five BAC Maxipreps, or 25 BAC Midipreps, or 100 BAC Minipreps.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format
Kit
Isolation Technology
Anion Exchange Resin
Sample Type
Bacterial Culture
Final Product Type
BAC DNA
For Use With (Application)
Sequencing, Cloning, Transformation, Transfection, In Vitro Transcription
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Product Line
PureLink™
Product Type
BAC Buffer Kit
Shipping Condition
Room Temperature
Target
BAC DNA, Plasmid DNA

Contents & Storage

The PureLink™ HiPure BAC Buffer Kit includes Resuspension Buffer (R3) (250 ml), Lysis Buffer (L7) (250 ml), Precipitation Buffer (N3) (250 ml), and RNase A (20 mg/ml) (5 ml). All components of the PureLink™ HiPure BAC Buffer Kit are shipped at room temperature. Store all components at room temperature upon receipt.

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