Invitrogen™

PureLink™ 96 HQ Plasmid DNA Purification Kit

Catalog number:  K210096

 Related applications:

Automated Nucleic Acid Purification

| DNA Extraction

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Description

The PureLink HQ 96 Plasmid DNA Purification Kit is designed for high-throughput isolation of high-quality plasmid DNA from bacterial cells. It can be used with a vacuum manifold or a centrifuge and is compatible with automated liquid handling workstations.

The PureLink HQ 96 Plasmid DNA Purification Kit offers the following advantages:
• Ability to isolate up to 10 µg plasmid DNA per well
• Designed to isolate high-quality plasmid DNA in 30-45 minutes
• Minimal genomic DNA contamination in the purified plasmid DNA
• Compatible with automated liquid handling workstations
• Reliable performance of the purified plasmid DNA in downstream applications such as mammalian transfection

Process overview
Bacterial cells are harvested and resuspended in Resuspension Buffer with RNase. The cells are lysed using an alkaline/SDS cell lysis procedure. The lysate is then neutralized and conditions are adjusted for subsequent binding. After lysate clarification using the PureLink HQ 96 Clarification Plate, the lysate is processed through the PureLink HQ 96 Binding Plate. The DNA binds to the silica-based membrane in the plate and impurities are removed by washing with Wash Buffer. The DNA is then eluted in low salt Elution Buffer or water.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

High Throughput Compatibility: High Throughput-Compatible
Sample Type (General): Bacteria
Downstream Application: Cloning, PCR, Sequencing, Transfection, Transformation
Final Product: Plasmid DNA
Isolation Technology: Silica Plate
Product Line: PureLink™
Product Size: 4 x 96 preps
Sample Type (Specific): Bacterial Culture
Shipping Condition: Room Temperature

Contents & storage

Sufficient reagents are provided in the kit to perform 384 (4 × 96) isolations.

Reagents include:
• Resuspension Buffer (R1)
• Lysis Buffer (L1)
• Neutralization/Binding Buffer (B1)
• Wash Buffer (W1)
• Elution Buffer; 10 mM Tris-HCl, pH 8.5 (E1)
• RNase A
• PureLink HQ 96 Clarification Plate
• PureLink HQ 96 Binding Plate
• Receiver Plate
• Adhesive Foil for plates

Documents

Manuals & protocols