PureLink™ Quick Gel Extraction and PCR Purification Combo Kit

Catalog number: K220001

Invitrogen™  Related applications: DNA Extraction

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Description

PureLink™ Quick Gel Extraction & PCR Purification Combo Kit offers the ability to perform both a gel extraction or a PCR purification a single kit.
The PureLink™ Quick Gel Extraction and PCR Purification Combo Kit is designed to purify DNA fragments from agarose gels. The simple procedure uses a silica-based spin cartridge to purify DNA fragments from 40 bp to 10 kb in < 30 minutes. It also provide all components to perform a rapid and efficient removal of short primers, dNTPs, enzymes, short-failed PCR products, and salts from PCR fagments >100 bp in less than 10 minutes. Isolated DNA using the PureLink™ kit is free of proteins, dye, and agarose, and is ready-to-use for a variety of applications, including automated fluorescent DNA sequencing, manual DNA sequencing, PCR, in vitro transcription, restriction mapping, cloning, and labeling. The kit contains all the buffers from the PureLink™ Quick Gel extraction kit and the PCR binding buffer from PureLink™ PCR Purification kit with Invitrogen branded lidded column.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Scale: Mini
System: PureLink™
Quantity: 1 kit
In-Gel Dye: Ethidium Bromide, SYBR® Safe
Product Size: 50 preps
Final Product: DNA
Yield (up to): 40 µg
DNA Fragment Size: Gel: purifies 40 bp to 10 kb, PCR: purifies >100 bp
Purification Time: 20 min
Elution Volume - max: 100 µl
Elution Volume - min: 30 µl
Isolation Technology: Silica Spin Column
Sample Type (General): Gel Samples
Downstream Application: PCR, Cloning, Next-Generation Sequencing, Sequencing, Nucleic Acid Labeling, In Vitro Transcription
Sample Type (Specific): PCR Products, DNA Fragments in Agarose Gel Slices
Purity or Quality Grade: Molecular Biology Grade
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Shipping Condition: Room Temperature

Contents & storage

The PureLink™ Quick Gel Extraction & PCR Purification Combo Kit includes sufficient PureLink™ columns, collection tubes, elution tubes, and reagents for 50 preps. Store components at room temperature. All reagents are guaranteed stable for 6 months when properly stored

Documents

Manuals & protocols

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