pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ Mach1™-T1R E. coli and PureLink™ Kit

Catalog number:  K252002

 Related applications: Gateway Cloning | PCR Cloning

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The pCR™8/GW/TOPO® TA Cloning Kit delivers simple 5 minute cloning and access to the Gateway® recombination cloning vector platform. Our pCR™8 TOPO® TA vector contains a minimal MCS, so you sequence more of your insert and less of the vector. And with our patented att sites, the pCR™8 vector is the simplest way to create an Entry Clone, which facilitates easy access via Gateway® recombination cloning to a variety of expression vectors without tedious sub-cloning, saving you valuable research time.

The pCR™8/GW/TOPO® TA Cloning Kit features:
• Fast growing E. coli that shortens cloning time
• Maximum convenience: includes plasmid miniprep kit
att sites for rapid recombination into any Gateway® destination vector
• 3'-T overhangs for direct ligation of Taq-amplified PCR products
• Sequencing primer sites within 55 bp of insertion site ensures you sequence more insert, less vector
• Spectinomycin resistance gene for robust selection in E. coli
• EcoR I sites flanking the PCR product insertion site for easy excision of inserts

This kit includes One Shot® Mach1™-T1R Chemically Competent E. coli and a PureLink® Quick Plasmid Miniprep Kit.
For Research Use Only. Not for use in diagnostic procedures.



Vector Type: pCR
Cloning Method: TOPO® with Gateway®
Product Line: Gateway®, Mach1™, One Shot®, PureLink™, TA Cloning®, TOPO®
Product Size: 20 preps

Contents & storage

pCR™8/GW/TOPO® Vector, dNTPs, Salt Solution, Sterile Water, Gateway Forward and Reverse Sequencing Primers, Control Template and PCR Primers; One Shot® TOP10 Chemically Competent E. coli Chemically Competent E. coli, S.O.C. Medium, and a pUC19 control plasmid. The PureLink™ Quick Plasmid Miniprep Kit contains Resuspension Buffer, RNase A, Lysis Buffer, Precipitation Buffer, Wash Buffer, TE Buffer, Spin Columns, and Wash and Recovery Tubes. Store all components of the PureLink™ kit at room temperature. Store the One Shot® E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.


Manuals & protocols