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Maxima H Minus Double-Stranded cDNA Synthesis Kit

Catalog number: K2561

Thermo Scientific™  Related applications: cDNA Libraries & Library Construction

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Description

Thermo Scientific Maxima H Minus Double-Stranded cDNA Synthesis Kit is a complete system for efficient synthesis of double-stranded cDNA from total RNA or mRNA. First- and second-strand cDNA synthesis reactions are performed in the same tube without the need for intermediate organic extraction or ethanol precipitation steps. This convenient one-tube format speeds up the synthesis procedure and maximizes cDNA recovery. The kit contains premixed components to reduce the number of pipetting steps necessary to complete the procedure.

Highlights

• Efficient synthesis of full length double-stranded cDNA
• Fast—procedure completed in less than 2 hours
• Convenient—premixed components
• Complete—includes all primers, controls and residual RNA removal reagents

Applications

• Full-length double-stranded blunt-end cDNA synthesis for cloning
• Double-stranded cDNA library construction
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Reverse Transcriptase: Maxima H Minus Reverse Transcriptase
Downstream Application: Cloning
Reaction Format: Separate Components
Sample Type (General): RNA
Optimal Reaction Temperature: 50-55° C
Product Line: Maxima
Product Size: 10 reactions

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