Thermo Scientific Maxima H Minus Double-Stranded cDNA Synthesis Kit is a complete system for efficient synthesis of double-stranded cDNA from total RNA or mRNA. First- and second-strand cDNA synthesis reactions are performed in the same tube without the need for intermediate organic extraction or ethanol precipitation steps. This convenient single-tube format speeds up the synthesis procedure and maximizes cDNA recovery. The kit contains pre-mixed components to reduce the number of pipetting steps necessary to complete the procedure.
Features of the Maxima H Minus Double-Stranded cDNA Synthesis Kit include: • Efficient synthesis of full-length double-stranded cDNA • Fast—procedure completed in less than two hours • Convenient—pre-mixed components • Complete—includes all primers, controls and residual RNA removal reagents
Applications • Full-length double-stranded blunt-end cDNA synthesis for cloning • Double-stranded cDNA library construction