Invitrogen™

Zero Blunt™ PCR Cloning Kit

Catalog number: K270020
Invitrogen™

Zero Blunt™ PCR Cloning Kit

Catalog number: K270020
Catalog Number
K270020
also known as K2700-20
Unit Size
20 reactions
Price (USD)
570.00
Your Price:
Availability
***
Quantity
Catalog Number
K270040
also known as K2700-40
Unit Size
40 reactions
Price (USD)
1,050.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
K270020
also known as K2700-20
20 reactions
570.00
Your Price:
***
K270040
also known as K2700-40
40 reactions
1,050.00
Your Price:
***
Product Overview
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The Zero Blunt® PCR Cloning Kit offers an easy method for high-efficiency (>80%) cloning of blunt-end PCR products amplified with proof-reading, thermostable DNA polymerases. The Zero Blunt® PCR Cloning Kit uses the multipurpose cloning vector pCR™-Blunt and ExpressLink™ T4 DNA Ligase to generate a ligation product in a five-minute, room-temperature ligation step.

Features of the Zero Blunt® Cloning® Kit with pCR™-Blunt vector:
Fast & convenient—5-minute, room-temperature ligation
EfficientccdB gene for positive selection results in >80% clones with correct insert
Flexible—choice of kanamycin or Zeocin™ resistance for flexible antibiotic selection

The pCR™-Blunt vector provides:
EcoR I sites flanking the PCR product insertion site for excision of inserts
• T7 promoter/primer site for in vitro RNA transcription and sequencing
• M13 forward and reverse primer sites for sequencing or PCR screening

How Zero Blunt® PCR Cloning Works
The Zero Blunt® PCR Cloning Kit is designed to clone blunt PCR fragments (or any blunt DNA fragment) with a low background of non-recombinants. The pCR™-Blunt vector contains the lethal E. coliccdB gene fused to the C-terminus of LacZα (Bernard et al., 1994). Ligation of a blunt PCR fragment disrupts expression of the lacZα-ccdB gene fusion permitting growth of only positive recombinants upon transformation. Cells that contain non-recombinant vector are killed when the transformation mixture is plated.

Kit Configurations
The Zero Blunt® PCR Cloning Kit is offered in a variety of configurations: with One Shot® TOPO10 Chemically Competent E. coli (K2700-20 and K2700-40) and without competent cells (K2750-20 and K2750-40) in 20- and 40- reaction kit sizes.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Bacterial or Yeast Strain
TOP10
Cloning Method
Blunt PCR
Vector
Blunt DNA Cloning Vectors
Product Line
Zero Blunt™
Product Type
PCR Cloning Kit
Promoter
T7
Quantity
20 reactions

Contents & Storage

Zero Blunt™ PCR cloning kits contain linearized pCR™-Blunt vector, ExpressLink™ T4 DNA ligase , 5X ExpressLink™ T4 DNA ligase buffer, control template, dNTPs, sterile water, and M13 forward and reverse primers. Competent cell kits contain One Shot™ chemically competent E. coli, S.O.C. medium, and a supercoiled control plasmid.

Store the One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.

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