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Zero Blunt™ PCR Cloning Kit
The Zero Blunt™ PCR Cloning Kit offers an easy method for high-efficiency (>80%) cloning of blunt-end PCR products amplified with자세히 알아보기
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보기 방식 변경
| 카탈로그 번호 | 수량 |
|---|---|
| K270040 | 40 Reactions |
| K270020 | 20 Reactions |
카탈로그 번호 K270040
제품 가격(KRW)
1,478,000
온라인 행사
Ends: 31-Dec-2025
1,738,000할인액 260,000 (15%)
Each
수량:
40 Reactions
제품 가격(KRW)
1,478,000
온라인 행사
Ends: 31-Dec-2025
1,738,000할인액 260,000 (15%)
Each
The Zero Blunt™ PCR Cloning Kit offers an easy method for high-efficiency (>80%) cloning of blunt-end PCR products amplified with proof-reading, thermostable DNA polymerases. The Zero Blunt™ PCR Cloning Kit uses the multipurpose cloning vector pCR™-Blunt and ExpressLink™ T4 DNA Ligase to generate a ligation product in a five-minute, room-temperature ligation step.
Features of the Zero Blunt™ Cloning™ Kit with pCR™-Blunt vector:
• Fast & convenient—5-minute, room-temperature ligation
• Efficient—ccdB gene for positive selection results in >80% clones with correct insert
• Flexible—choice of kanamycin or Zeocin™ resistance for flexible antibiotic selection
The pCR™-Blunt vector provides:
• EcoR I sites flanking the PCR product insertion site for excision of inserts
• T7 promoter/primer site for in vitro RNA transcription and sequencing
• M13 forward and reverse primer sites for sequencing or PCR screening
How Zero Blunt™ PCR Cloning Works
The Zero Blunt™ PCR Cloning Kit is designed to clone blunt PCR fragments (or any blunt DNA fragment) with a low background of non-recombinants. The pCR™-Blunt vector contains the lethal E. coliccdB gene fused to the C-terminus of LacZα (Bernard et al., 1994). Ligation of a blunt PCR fragment disrupts expression of the lacZα-ccdB gene fusion permitting growth of only positive recombinants upon transformation. Cells that contain non-recombinant vector are killed when the transformation mixture is plated.
Kit Configurations
The Zero Blunt™ PCR Cloning Kit is offered in a variety of configurations: with One Shot™ TOPO10 Chemically Competent E. coli (K2700-20 and K2700-40) and without competent cells (K2750-20 and K2750-40) in 20- and 40- reaction kit sizes.
Features of the Zero Blunt™ Cloning™ Kit with pCR™-Blunt vector:
• Fast & convenient—5-minute, room-temperature ligation
• Efficient—ccdB gene for positive selection results in >80% clones with correct insert
• Flexible—choice of kanamycin or Zeocin™ resistance for flexible antibiotic selection
The pCR™-Blunt vector provides:
• EcoR I sites flanking the PCR product insertion site for excision of inserts
• T7 promoter/primer site for in vitro RNA transcription and sequencing
• M13 forward and reverse primer sites for sequencing or PCR screening
How Zero Blunt™ PCR Cloning Works
The Zero Blunt™ PCR Cloning Kit is designed to clone blunt PCR fragments (or any blunt DNA fragment) with a low background of non-recombinants. The pCR™-Blunt vector contains the lethal E. coliccdB gene fused to the C-terminus of LacZα (Bernard et al., 1994). Ligation of a blunt PCR fragment disrupts expression of the lacZα-ccdB gene fusion permitting growth of only positive recombinants upon transformation. Cells that contain non-recombinant vector are killed when the transformation mixture is plated.
Kit Configurations
The Zero Blunt™ PCR Cloning Kit is offered in a variety of configurations: with One Shot™ TOPO10 Chemically Competent E. coli (K2700-20 and K2700-40) and without competent cells (K2750-20 and K2750-40) in 20- and 40- reaction kit sizes.
For Research Use Only. Not for use in diagnostic procedures.
사양
박테리아 또는 효모 균주TOP10
클로닝 방법Blunt PCR
제품라인Zero Blunt
제품 유형PCR Cloning Kit
프로모터T7
수량40 Reactions
벡터Blunt DNA Cloning Vectors
형식Kit
Unit SizeEach
구성 및 보관
Zero Blunt™ PCR cloning kits contain linearized pCR™-Blunt vector, ExpressLink™ T4 DNA ligase , 5X ExpressLink™ T4 DNA ligase buffer, control template, dNTPs, sterile water, and M13 forward and reverse primers. Competent cell kits contain One Shot™ chemically competent E. coli, S.O.C. medium, and a supercoiled control plasmid.
Store the One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
Store the One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.