Search
Search
View additional product information for MagJET mRNA Enrichment Kit - FAQs (K2811)
10 product FAQs found
We would highly recommend using the provided Elution Buffer in the elution step for best results. You may use nuclease-free water; however, it may affect the yield of the nucleic acids during purification and their stability during storage.
Thermo Scientific offers MagJET Separation Rack of different formats for 1.5 mL tubes (Cat. Nos. MR01, MR02), 50 mL tubes (Cat. No. MR04), and 96-well plates (Cat. No. MR03). You may use other compatible magnetic racks available in the market as well.
No, we do not offer a sample size for MagJET kits.
No, we do not offer GeneJET columns or MagJET magnetic beads separately at this time.
Yes, we recommend using MagJET mRNA Enrichment Kit for NGS.
No, the kit is suitable for mRNA isolation from previously purified total RNA samples.
No, manufactured protocols are locked for editing, but they can be renamed using the ‘save as' function and edited as a new version.
For nucleic acid extraction, we recommend using our MagMAX kits and MagJET kits. Other magnetic beads should work well as long as they are 0.5 to 10 µm in size. However, you will have to write your own KingFisher protocols using the Thermo Scientific BindIt software and validate/optimize the protocol on your own.
Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.
Yes, this can be done by sample splitting. Set the protocol so that the magnet will collect samples from multiple wells before continuing to the next step. Be sure not to overload.
Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.
Yes, reaction volumes can be scaled up, and all other volumes (i.e. wash volumes) will have to be increased by the same fold difference. It is generally not recommended to scale up reaction volumes more than 2-fold unless the plate's volume capacity will allow it.