Invitrogen™

      Zero Blunt™ TOPO™ PCR Cloning Kit, with One Shot™ MAX Efficiency DH5α-T1R E. coli

      Zero Blunt™ TOPO™ PCR Cloning Kit, with One Shot™ MAX Efficiency DH5α-T1<sup>R</sup> <i>E. coli</i>

      Catalog number:  K282040

       Related applications: PCR Cloning

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      Description

      Zero Blunt® TOPO® PCR Cloning Kits offers the fastest and easiest method for high-efficiency (cloning of blunt-end PCR products amplified with proofreading thermostable polymerases. The kits include linearized and topoisomerase I-activated pCR™-Blunt II-TOPO® Vector for 5-minute benchtop ligations without ligase (1). The pCR™-Blunt II-TOPO® Vector also features:

      • ccdB gene for positive selection (2)
      • EcoR I sites flanking the PCR product insertion site for easy excision of inserts
      • Kanamycin and Zeocin™ resistance genes for your choice of selection in E. coli
      • SP6 promoter/primer site for in vitro RNA transcription and sequencing
      • M13 forward and reverse primer sites for sequencing or PCR screening

      Selected Zero Blunt® TOPO® PCR Cloning Kits are available in combo kits combined with a PureLink™ Quick Plasmid Miniprep Kit (50 preps) for fast plasmid purification of your TOPO®-cloned inserts for downstream analysis.
      For Research Use Only. Not for use in diagnostic procedures.

      Specifications

      Vector Type: Blunt DNA Cloning Vectors
      Cloning Method: Blunt TOPO®
      Bacterial or Yeast Strain: DH5α™
      Product Line: DH5a™, MAX Efficiency®, One Shot®, TOPO®, Zero Blunt®
      Product Size: 50 reactions

      Contents & storage

      Box 1:
      • Linearized and topoisomerase I-activated pCR™Blunt II-TOPO® vector
      • Salt solution
      • dNTPs
      • Control template
      • M13 forward and reverse primers
      • Sterile water

      Store at -5 to -30°C.
      All reagents are stable for 6 months when properly stored.

      Box 2:
      • One Shot® Chemically Competent or Electrocomp™ E. coli
      • S.O.C. medium
      • Supercoiled pUC19 control plasmid

      Store at -68 to -85°C.

      Documents

      Manuals & protocols