Invitrogen™

Zero Blunt™ TOPO PCR Cloning Kit for Subcloning

Catalog number:  K283020

Related applications:

Chromatin Biology

| Methylation Analysis

| PCR Cloning

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Description

The Zero Blunt® TOPO® PCR Cloning Kit for subcloning combines the unique Zero Background™ technology with the pCR™- Blunt II-TOPO® vector (Figure 7) to allow easy, high-efficiency cloning of blunt-end PCR products.
• Unique technology to minimize background
• Get your clone—95% efficiency
• Multiple primer sites (T7, T3, M13F, M13R) make sequence analysis or PCR easy and convenient
• Kanamycin and Zeocin resistance genes for your choice of selection
• Convenient, validated restriction sites in MCS for further subcloning
• Includes MachI™ T1R competent cells for fast miniprep and shortens cloning time
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Bacterial or Yeast Strain: Mach1™
Cloning Method: Blunt TOPO®
Product Line: One Shot™
Vector: Blunt DNA Cloning Vectors

Contents & storage

Box 1:
• Linearized and topoisomerase I-activated pCR™Blunt II-TOPO® vector
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

Box 2:
• One Shot® Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid

Store at -68 to -85°C.