The Zero Blunt® TOPO® PCR Cloning Kit for subcloning combines the unique Zero Background™ technology with the pCR™- Blunt II-TOPO® vector (Figure 7) to allow easy, high-efficiency cloning of blunt-end PCR products. • Unique technology to minimize background • Get your clone—95% efficiency • Multiple primer sites (T7, T3, M13F, M13R) make sequence analysis or PCR easy and convenient • Kanamycin and Zeocin resistance genes for your choice of selection • Convenient, validated restriction sites in MCS for further subcloning • Includes MachI™ T1R competent cells for fast miniprep and shortens cloning time
For Research Use Only. Not for use in diagnostic procedures.