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Zero Blunt™ TOPO PCR Cloning Kit for Subcloning
The Zero Blunt™ TOPO™ PCR Cloning Kit for subcloning combines the unique Zero Background™ technology with the pCR™- Blunt II-TOPO™Read more
Catalog number K283020
also known as K2830-20
Price (USD)/ Each
810.65
Online Exclusive
854.00Save 43.35 (5%)
Quantity:
25 Reactions
Price (USD)/ Each
810.65
Online Exclusive
854.00Save 43.35 (5%)
The Zero Blunt™ TOPO™ PCR Cloning Kit for subcloning combines the unique Zero Background™ technology with the pCR™- Blunt II-TOPO™ vector (Figure 7) to allow easy, high-efficiency cloning of blunt-end PCR products.
• Unique technology to minimize background
• Get your clone—95% efficiency
• Multiple primer sites (T7, T3, M13F, M13R) make sequence analysis or PCR easy and convenient
• Kanamycin and Zeocin resistance genes for your choice of selection
• Convenient, validated restriction sites in MCS for further subcloning
• Includes MachI™ T1R competent cells for fast miniprep and shortens cloning time
• Unique technology to minimize background
• Get your clone—95% efficiency
• Multiple primer sites (T7, T3, M13F, M13R) make sequence analysis or PCR easy and convenient
• Kanamycin and Zeocin resistance genes for your choice of selection
• Convenient, validated restriction sites in MCS for further subcloning
• Includes MachI™ T1R competent cells for fast miniprep and shortens cloning time
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Bacterial or Yeast StrainMach1™
Cloning MethodBlunt TOPO™
FormatKit
For Use With (Application)Chromatin Biology
Product LineOne Shot™
Product TypePCR Cloning Kit
Quantity25 Reactions
VectorBlunt DNA Cloning Vectors
Unit SizeEach
Contents & Storage
Box 1:
• Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Sterile water
Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.
Box 2:
• One Shot™ Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid
Store at -68 to -85°C.
• Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Sterile water
Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.
Box 2:
• One Shot™ Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid
Store at -68 to -85°C.