Zero Blunt™ TOPO PCR Cloning Kit for Subcloning

      Zero Blunt™ TOPO PCR Cloning Kit for Subcloning

      Catalog number:  K283020

      Invitrogen™  Related applications: Chromatin Biology | Methylation Analysis | PCR Cloning

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      Description

      The Zero Blunt® TOPO® PCR Cloning Kit for subcloning combines the unique Zero Background™ technology with the pCR™- Blunt II-TOPO® vector (Figure 7) to allow easy, high-efficiency cloning of blunt-end PCR products.
      • Unique technology to minimize background
      • Get your clone—95% efficiency
      • Multiple primer sites (T7, T3, M13F, M13R) make sequence analysis or PCR easy and convenient
      • Kanamycin and Zeocin resistance genes for your choice of selection
      • Convenient, validated restriction sites in MCS for further subcloning
      • Includes MachI™ T1R competent cells for fast miniprep and shortens cloning time
      For Research Use Only. Not for use in diagnostic procedures.

      Specifications

      Vector Type: Blunt DNA Cloning Vectors
      Cloning Method: Blunt TOPO®
      Bacterial or Yeast Strain: Mach1™
      Product Line: Mach1™, One Shot®, TOPO®, Zero Background™, Zero Blunt®
      Product Size: 25 reactions

      Contents & storage

      Box 1:
      • Linearized and topoisomerase I-activated pCR™Blunt II-TOPO® vector
      • Salt solution
      • dNTPs
      • Control template
      • M13 forward and reverse primers
      • Sterile water

      Store at -5 to -30°C.
      All reagents are stable for 6 months when properly stored.

      Box 2:
      • One Shot® Chemically Competent or Electrocomp™ E. coli
      • S.O.C. medium
      • Supercoiled pUC19 control plasmid

      Store at -68 to -85°C.

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