Invitrogen™

Zero Blunt™ TOPO PCR Cloning Kit for Subcloning

Catalog number: K283020
Invitrogen™

Zero Blunt™ TOPO PCR Cloning Kit for Subcloning

Catalog number: K283020
Catalog Number
K283020
also known as K2830-20
Unit Size
25 reactions
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
K283020
also known as K2830-20
25 reactions
-
Product Overview
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The Zero Blunt™ TOPO™ PCR Cloning Kit for subcloning combines the unique Zero Background™ technology with the pCR™- Blunt II-TOPO™ vector (Figure 7) to allow easy, high-efficiency cloning of blunt-end PCR products.
• Unique technology to minimize background
• Get your clone—95% efficiency
• Multiple primer sites (T7, T3, M13F, M13R) make sequence analysis or PCR easy and convenient
• Kanamycin and Zeocin resistance genes for your choice of selection
• Convenient, validated restriction sites in MCS for further subcloning
• Includes MachI™ T1R competent cells for fast miniprep and shortens cloning time
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Bacterial or Yeast Strain
Mach1™
Cloning Method
Blunt TOPO™
Format
Kit
For Use With (Application)
Chromatin Biology
Product Line
One Shot™
Product Type
PCR Cloning Kit
Quantity
25 Reactions
Vector
Blunt DNA Cloning Vectors

Contents & Storage

Box 1:
• Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

Box 2:
• One Shot™ Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid

Store at -68 to -85°C.

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