Invitrogen™
Zero Blunt™ TOPO PCR Cloning Kit for Subcloning
Catalog number: K283020
Related applications:Description
The Zero Blunt® TOPO® PCR Cloning Kit for subcloning combines the unique Zero Background™ technology with the pCR™- Blunt II-TOPO® vector (Figure 7) to allow easy, high-efficiency cloning of blunt-end PCR products.• Unique technology to minimize background
• Get your clone—95% efficiency
• Multiple primer sites (T7, T3, M13F, M13R) make sequence analysis or PCR easy and convenient
• Kanamycin and Zeocin resistance genes for your choice of selection
• Convenient, validated restriction sites in MCS for further subcloning
• Includes MachI™ T1R competent cells for fast miniprep and shortens cloning time
For Research Use Only. Not for use in diagnostic procedures.
Figures
Specifications
| Bacterial or Yeast Strain: | Mach1™ |
|---|---|
| Cloning Method: | Blunt TOPO® |
| Product Line: | One Shot™ |
| Vector: | Blunt DNA Cloning Vectors |
Contents & storage
Box 1:
• Linearized and topoisomerase I-activated pCR™Blunt II-TOPO® vector
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Sterile water
Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.
Box 2:
• One Shot® Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid
Store at -68 to -85°C.
• Linearized and topoisomerase I-activated pCR™Blunt II-TOPO® vector
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Sterile water
Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.
Box 2:
• One Shot® Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid
Store at -68 to -85°C.
