The Zero Blunt™ TOPO™ PCR Cloning Kit for subcloning combines the unique Zero Background™ technology with the pCR™- Blunt II-TOPO™ vector (Figure 7) to allow easy, high-efficiency cloning of blunt-end PCR products. • Unique technology to minimize background • Get your clone—95% efficiency • Multiple primer sites (T7, T3, M13F, M13R) make sequence analysis or PCR easy and convenient • Kanamycin and Zeocin resistance genes for your choice of selection • Convenient, validated restriction sites in MCS for further subcloning • Includes MachI™ T1R competent cells for fast miniprep and shortens cloning time
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Bacterial or Yeast Strain
Mach1™
Cloning Method
Blunt TOPO™
Format
Kit
For Use With (Application)
Chromatin Biology
Product Line
One Shot™
Product Type
PCR Cloning Kit
Quantity
25 Reactions
Vector
Blunt DNA Cloning Vectors
Contents & Storage
Box 1: • Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Sterile water
Store at -5 to -30°C. All reagents are stable for 6 months when properly stored.
Box 2: • One Shot™ Chemically Competent or Electrocomp™ E. coli • S.O.C. medium • Supercoiled pUC19 control plasmid