Zero Blunt® TOPO® PCR Cloning Kits offers the fastest and easiest method for high-efficiency (cloning of blunt-end PCR products amplified with proofreading thermostable polymerases. The kits include linearized and topoisomerase I-activated pCR™-Blunt II-TOPO® Vector for 5-minute benchtop ligations without ligase (1). The pCR™-Blunt II-TOPO® Vector also features:
• ccdB gene for positive selection (2) • EcoR I sites flanking the PCR product insertion site for easy excision of inserts • Kanamycin and Zeocin™ resistance genes for your choice of selection in E. coli • SP6 promoter/primer site for in vitro RNA transcription and sequencing • M13 forward and reverse primer sites for sequencing or PCR screening
Selected Zero Blunt® TOPO® PCR Cloning Kits are available in combo kits combined with a PureLink™ Quick Plasmid Miniprep Kit (50 preps) for fast plasmid purification of your TOPO®-cloned inserts for downstream analysis.
For Research Use Only. Not for use in diagnostic procedures.
Bacterial or Yeast Strain
Blunt DNA Cloning Vectors
Kit, PCR Cloning
Contents & Storage
Box 1: • Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Sterile water
Store at -5 to -30°C. All reagents are stable for 6 months when properly stored.
Box 2: • One Shot™ Chemically Competent or Electrocomp™ E. coli • S.O.C. medium • Supercoiled pUC19 control plasmid