Kit de purificación de PCR PureLink™
Kit de purificación de PCR PureLink™
Citas y referencias (4)
Invitrogen™

Kit de purificación de PCR PureLink™

Se elimina la necesidad de una tediosa purificación de gel
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Número de catálogoCantidad
K310002250 preparaciones
K31000150 preparaciones
Número de catálogo K310002
Precio (MXN)
-
Cantidad:
250 preparaciones
The PureLink PCR Purification Kit provides a more convenient, rapid, and high-yielding purification process compared to other companies' kits. After PCR amplification, purification of the PCR amplicons is required for many common downstream applications, including enzyme digestion, ligation, sequencing, and labeling experiments, to prevent enzymes (polymerase, restriction enzyme) and reaction components (dNTPs, primers, buffers, salts) from carrying over, contaminating, and negatively influencing the downstream experiments.

Advantages of the PureLink PCR Purification Kit include:

  • 4X binding capacity compared to Company Q; 40 μg for the PureLink kit versus 10 μg from other companies' kits
  • No need for tedious sample pH adjustments necessary with other kits
  • Convenient and rapid protocol, completed in less than 10 minutes
  • Optional size-selection buffers included for selectively purifying 300–600 bp fragments

The PureLink PCR Purification Kit is based on the selective binding of dsDNA to silica-based membrane in the presence of chaotropic salts. Simply mix the PCR product with Binding Buffer and apply to the PureLink Spin Column. The dsDNA binds to the silica-based membrane in the column. Remove impurities by thorough washing with Wash Buffer. To purify the DNA, elute the dsDNA in low-salt Elution Buffer or water.The purified PCR product is suitable for automated fluorescent DNA sequencing, restriction enzyme digestion, and cloning.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Volumen de elución máx50 μL
Tipo de producto finalPCR Amplicon
FormatoKit
Compatibilidad de alto rendimientoNo compatible con alto rendimiento (manual)
Tecnología de aislamientoColumna de centrifugación de sílice
Tiempo de purificación15 min.
Cantidad250 preparaciones
Tiempo de actividad10 min
Tipo de muestraProducto de PCR
Condiciones de envíoTemperatura ambiente
Volumen de material de partida≤100 μL PCR product, or ≤40 μg dsDNA
Producción40 μg (Binding capacity)
Para utilizar con (aplicación)Secuenciación de última generación (NGS), Clonación, PCR estándar, Nucleic Acid Labeling, Cloning
Unit SizeEach
Contenido y almacenamiento
For better long-term performance, it is recommended to store the purification columns at 2°C to 8°C.

Preguntas frecuentes

Can I use the PureLink PCR Purification Kit to clean up restriction digested plasmid DNA?

Yes, you can use our kit to clean up restriction digested plasmid DNA.

Can I use the PureLink PCR Purification Kit to do a gel purification?

The buffers and procedure are different for the PureLink PCR Purification Kit and PureLink Gel Purification Kit. Therefore, you can swap the column, but the right buffer and protocol must be used.

What are the minimum and maximum size ranges for DNA fragment cleanup using the PureLink PCR Clean-Up Kit?

The size range is between 100 bp and 12 kb. Our kit comes with two buffers, Binding Buffer (B2) and Binding Buffer HC (B3). The Binding Buffer HC (B3) eliminates primer-dimers and short failed PCR products that are smaller than 300 bp.

Citations & References (4)

Citations & References
Abstract
A virulence and antimicrobial resistance DNA microarray detects a high frequency of virulence genes in Escherichia coli isolates from Great Lakes recreational waters.
Authors:Hamelin K, Bruant G, El-Shaarawi A, Hill S, Edge TA, Bekal S, Fairbrother JM, Harel J, Maynard C, Masson L, Brousseau R,
Journal:Appl Environ Microbiol
PubMed ID:16751532
'Escherichia coli is generally described as a commensal species with occasional pathogenic strains. Due to technological limitations, there is currently little information concerning the prevalence of pathogenic E. coli strains in the environment. For the first time, using a DNA microarray capable of detecting all currently described virulence genes and ... More
The incidence and clinical significance of nucleophosmin mutations in childhood AML.
Authors:Brown P, McIntyre E, Rau R, Meshinchi S, Lacayo N, Dahl G, Alonzo TA, Chang M, Arceci RJ, Small D,
Journal:Blood
PubMed ID:17440048
Frameshift mutations in exon 12 of the nucleophosmin gene (NPM1) result in aberrant cytoplasmic localization of the NPM protein (NPMc(+)) and occur in 25% to 35% of adult acute myeloid leukemia (AML). In adults with AML, NPMc(+) has been associated with normal karyotype, FLT3/ITD mutations, high remission induction rates, and ... More
Accuracy of six antimicrobial susceptibility methods for testing linezolid against staphylococci and enterococci.
Authors:Tenover FC, Williams PP, Stocker S, Thompson A, Clark LA, Limbago B, Carey RB, Poppe SM, Shinabarger D, McGowan JE,
Journal:J Clin Microbiol
PubMed ID:17634301
A challenge panel of enterococci (n = 50) and staphylococci (n = 50), including 17 and 15 isolates that were nonsusceptible to linezolid, respectively, were tested with the Clinical and Laboratory Standards Institute broth microdilution and disk diffusion reference methods. In addition, all 100 isolates were tested in parallel by ... More
Occurrence of virulence and antimicrobial resistance genes in Escherichia coli isolates from different aquatic ecosystems within the St. Clair River and Detroit River areas.
Authors:Hamelin K, Bruant G, El-Shaarawi A, Hill S, Edge TA, Fairbrother J, Harel J, Maynard C, Masson L, Brousseau R,
Journal:Appl Environ Microbiol
PubMed ID:17085696
Although the number of Escherichia coli bacteria in surface waters can differ greatly between locations, relatively little is known about the distribution of E. coli pathotypes in surface waters used as sources for drinking or recreation. DNA microarray technology is a suitable tool for this type of study due to ... More