PureLink® PCR Micro Kit

Catalog number: K310250

Invitrogen™  Related applications: DNA Extraction

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Description

PureLink® PCR Micro Kits are ideal for purifying and concentrating DNA from low-yielding PCR or restriction digestion reactions. These highly purified DNA fragments and amplicons provide excellent results in downstream applications such as DNA sequencing, restriction digestion, cloning, PCR, and labeling. PureLink® PCR Micro Kits provide a simple and rapid method for purifying and concentrating DNA fragments ranging in size from 100 bp-12 Kb. The unique micro-nozzle design allows up to 95% recovery of DNA fragments with as little as a 5 µl elution volume. With less than a 10% coefficient of variance in the elution volume you can be sure that no ethanol is carried over from the wash steps giving you the best chance of success in downstream applications. Removal of primers, dNTPs, enzymes, and salts exceeds 98.5%. This kit can also efficiently remove unincorporated dye-labeled nucleotides from a cDNA labeling reaction.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Sample Type (General): Enzymatic Reactions (e.g. PCR)
Downstream Application: Nucleic Acid Labeling, PCR, Cloning, Sequencing
Quantity: 250 columns
Product Size: 250 preps
Separation Range: 100 bp to 12 kb
Purification Time: 10 min
Elution Volume - max: 20 µl
Elution Volume - min: 5 µl
Isolation Technology: Silica Spin Column
Sample Type (Specific): DNA (Restriction Enzyme-Digested), cDNA Labeling Reactions, PCR Products
Starting Material (Volume): 125 µl
Shipping Condition: Room Temperature

Contents & storage

Kit includes columns, binding and wash buffers, recovery tubes, and the manual. Store at room temperature.

Documents

Manuals & protocols

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