A protein kinase encoded by the t complex responder gene causes non-mendelian inheritance
AuthorsBERNHARD G. HERRMANN, BIRGIT KOSCHORZ, KARIN WERTZ, K. JOHN MCLAUGHLIN* & ANDREAS KISPERT
JournalNature
PubMed ID10647005
'Males heterozygous for the t-haplotype form of mouse chromosome 17 preferentially transmit the t-chromosome to their progeny. Several distorter/sterility loci carried on the t-haplotype together impair flagellar function in all spermatozoa whereas the responder, Tcr, rescues t-sperm but not wild-type sperm. Thus, t-sperm have anadvantage over wild-type sperm in fertilizing ... More
The cathepsin B of Toxoplasma gondii, toxopain-1, is critical for parasite invasion and rhoptry protein processing.
Authors Que Xuchu; Ngo Huân; Lawton Jeffrey; Gray Mary; Liu Qing; Engel Juan; Brinen Linda; Ghosh Partho; Joiner Keith A; Reed Sharon L;
JournalJ Biol Chem
PubMed ID12000756
'Cysteine proteinases play a major role in invasion and intracellular survival of a number of pathogenic parasites. We cloned a single copy gene, tgcp1, from Toxoplasma gondii and refolded recombinant enzyme to yield active proteinase. Substrate specificity of the enzyme and homology modeling identified the proteinase as a cathepsin B. ... More
Bacterial cell killing mediated by topoisomerase I DNA cleavage activity.
'DNA topoisomerases are important clinical targets for antibacterial and anticancer therapy. At least one type IA DNA topoisomerase can be found in every bacterium, making it a logical target for antibacterial agents that can convert the enzyme into poison by trapping its covalent complex with DNA. However, it has not ... More
Degradation of corn fiber by Clostridium cellulovorans cellulases and hemicellulases and contribution of scaffolding protein CbpA.
AuthorsKoukiekolo R, Cho HY, Kosugi A, Inui M, Yukawa H, Doi RH,
JournalAppl Environ Microbiol
PubMed ID16000754
'Clostridium cellulovorans, an anaerobic bacterium, degrades native substrates efficiently by producing an extracellular enzyme complex called the cellulosome. All cellulosomal enzyme subunits contain dockerin domains that can bind to hydrophobic domains termed cohesins which are repeated nine times in CbpA, the nonenzymatic scaffolding protein of C. cellulovorans cellulosomes. In this ... More
Factor B and the mitochondrial ATP synthase complex.
Authors Belogrudov Grigory I; Hatefi Youssef;
JournalJ Biol Chem
PubMed ID11744738
Factor B is a subunit of the mammalian ATP synthase complex, whose existence has been controversial. This paper describes the molecular and functional properties of a recombinant human factor B, which when added to bovine submitochondrial particles depleted of their factor B restores the energy coupling activity of the ATP ... More
Understanding the polymerization mechanism of glycoside-hydrolase family 70 glucansucrases.
AuthorsMoulis C, Joucla G, Harrison D, Fabre E, Potocki-Veronese G, Monsan P, Remaud-Simeon M,
JournalJ Biol Chem
PubMed ID16864576
Glucan formation catalyzed by two GH-family 70 enzymes, Leuconostoc mesenteroides NRRL B-512F dextransucrase and L. mesenteroides NRRL B-1355 alternansucrase, was investigated by combining biochemical and kinetic characterization of the recombinant enzymes and their respective products. Using HPAEC analysis, we showed that two molecules act as initiator of polymerization: sucrose itself ... More
Role of the two catalytic domains of DSR-E dextransucrase and their involvement in the formation of highly alpha-1,2 branched dextran.
AuthorsFabre E, Bozonnet S, Arcache A, Willemot RM, Vignon M, Monsan P, Remaud-Simeon M,
JournalJ Bacteriol
PubMed ID15601714
The dsrE gene from Leuconostoc mesenteroides NRRL B-1299 was shown to encode a very large protein with two potentially active catalytic domains (CD1 and CD2) separated by a glucan binding domain (GBD). From sequence analysis, DSR-E was classified in glucoside hydrolase family 70, where it is the only enzyme to ... More