ViraPower™ II Lentiviral N-Lumio™ Gateway® Expression System

Catalog number: K37120

Invitrogen™  Related applications: Gateway Cloning | Protein Expression

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Description

To efficiently deliver and monitor expression of your gene in live cells, choose a Lentivirus expression vector to fuse your protein with Lumio™ and V5 epitope tag. Lumio™ technology is designed for simple fluorescent detection of recombinant protein. The Lumio™ tag is a six amino acid sequence that binds a fluorescent substrate, allowing you to visually detect your protein of interest in mammalian cells. Choose from either the C-terminal or N-terminal fusion tagged vectors for optimal expression. These Lentiviral Lumio™ Reporter vectors are configured for making high-titer Lentiviral virus preps for efficient viral delivery to both dividing and non-dividing cells and selection with blasticidin.The pLenti6.2/C,N-Lumio™/V5-DEST vectors provide:

• CMV promoter for high-level expression of your gene
• PGK promoter for long-term stable expression of the Blasticidin (bsd)-resistance marker
• Lumio™ tag for accurate in vivo and in vitro protein detection
• Sites for rapid recombinational cloning using Gateway® Technology
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Promoter: CMV
Key Function: Viral Expression
Protein Tag or Fusion: V5 Epitope Tag, Lumio
Delivery Method: Lentiviral
Cloning Method: Gateway®
Selection Agent (Eukaryotic): Blasticidin
Vector Type: pLenti
Product Size: 1 kit
Constitutive or Inducible System: Constitutive

Contents & storage

Each ViraPower™ II Lentiviral Lumio™ Gateway® Expression System includes a vector kit, a Lumio™ In Cell Detection kit, 293FT cells, competent e. coli, and a Lentiviral Support Kit. The vector kit contains 6 µg of either the pLenti6.2/C-Lumio™/V5-DEST or pLenti6.2/N-Lumio™/V5-DEST vector as lyophilized plasmid DNA. The kit also includes a pLenti6.2/Lumio™/V5-GW/lacZ expression control plasmid. Both vectors should be stored at -20oC when resuspended and are guaranteed stable for 6 months. The Lumio™ Green In-Cell Detection Kit includes 40 µl of Lumio™ Green fluorescent reagent as a 2 mM stock solution in DMSO. When stored at -20°C, the reagent is guaranteed stable for 6 months. A 20 reaction kit of One Shot® Stbl3™ Chemically competent Cells is included and should be stored at -80°C. One kit consisting of 3 vials (3 x 106 cells each) of 293FT Cells are included and should be stored in liquid nitrogen for optimal viability. The ViraPower™ Bsd Lentiviral Support Kit is a single box containing 195 µg lyophilized ViraPower™ Lentiviral Packaging Mix, 750 µl Lipofectamine™ 2000 Reagent, and 50 mg of Blasticidin selection agent. Store Lipofectamine™ 2000 Reagent at +4°C. Store the ViraPower™ Lentiviral Packaging Mix and Blasticidin at -20°C once they have been resuspended. All components are guaranteed stable for 6 months when properly stored.

Documents

Manuals & protocols