The DES® TOPO® TA Expression Kit offers one-step cloning of PCR products directly into an inducible DES® expression vector. The kit uses the linearized, topoisomerase I-activated pMT/V5-His-TOPO® vector for fast and easy cloning and subsequent high-level expression. Topoisomerase activation of this vector allows PCR products to be ligated in just 5 minutes on your bench top to produce >85%recombinants. The pMT/V5-His-TOPO® vector has all of the features of the pMT/V5-His vector to simplify protein expression, detection, and purification of recombinant proteins in Drosophila S2 cells.
For Research Use Only. Not for use in diagnostic procedures.
Each DES® TOPO® TA Expression Kit contains two boxes. The DES® TOPO® TA box contains 200 ng of linearized and topoisomerase-activated pMT/V5-His- TOPO® vector, dNTPs, salt solution, control PCR template and primers, forward and reverse primers for sequencing and PCR screening, and a control expression plasmid (pMT/lacZ). Store at -20°C. The One Shot® box contains high-efficiency, single-use 50 µl aliquots of competent TOP10 E. coli cells, S.O.C. medium, and pUC19 supercoiled plasmid control. Store at -80°C. All reagents are guaranteed stable for 6 months when properly stored.