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Invitrogen™

pYES2.1 TOPO™ TA Yeast Expression Kit

Catalog number:  K415001

Related applications:

Protein Expression

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Description

The pYES2.1 TOPO TA Expression Kit offers direct cloning of a Taq-amplified PCR product into a Saccharomyces cerevisiae expression vector. The kit uses the linearized, topoisomerase I-activated pYES2.1/V5-His-TOPO ¤ vector for 5-minute cloning on your bench top and produces >85% recombinants. The pYES2.1/V5- His-TOPO® vector features:

• 2µ origin of replication for high-copy maintenance gene for auxotrophic selection on economical minimal media
• C-terminal V5 epitope and polyhistidine (6xHis) tags for efficient detection and purification

Specifications

Promoter: GAL1
Antibiotic Resistance Bacterial: Ampicillin (AmpR)
Product Line: TOPO™, YES™
Protein Tag: His Tag (6x), V5 Epitope Tag
Cloning Method: TOPO™-TA
Vector: pYES, TOPO-TA Cloning Vectors

Contents & storage

Each pYES2.1 TOPO™ TA Expression Kit contains two boxes. The pYES2.1 TOPO™ TA box contains all of the reagents necessary for ligation, including 200 ng of topoisomerase I-activated pYES2.1/V5-His-TOPO™ vector, sterile water, dNTPs, 10X PCR buffer, salt solution, control template and primers, GAL1 Forward and V5 C-term Reverse Primers for sequencing or PCR screening, and an expression control plasmid. Store at -20°C. The One Shot™ Box contains all of the reagents necessary for transformation, including twenty-one 50 µl aliquots of chemically competent TOP10F´ E. coli, S.O.C. medium, and supercoiled plasmid control pUC19. Store at -80°C. All components are guaranteed stable at the indicated temperature for 6 months when properly stored.