pBAD202 Directional TOPO™ Expression Kit

Catalog number:  K420201

 Related applications:

Protein Expression

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Using restriction enzymes to clone your gene into an expression vector often forces you to compromise the final sequence of your insert (Figure 1A), especially when there are no useful restriction sites close to your genes coding sequence. This may result in suboptimal spacing of expression elements or incorporation of non-native amino acid residues, which can reduce your expression levels and/or cause the production of non-functional protein.

In addition to being a more effective way to clone, TOPO® Cloning eliminates these potential expression problems. TOPO® Expression Vectors enable you to insert the exact DNA sequence you require simply by performing PCR with appropriately designed primers. Your PCR product is cloned at a high efficiency in only five minutes into a topoisomerase I-activated expression vector. The resulting recombinant expression vector contains your exact DNA sequence without any non-coding regions (Figure 1B).

Many of Invitrogen's powerful expression vectors are available adapted for one-step TOPO® cloning and expression of PCR products. In addition, several expression vectors are now adapted for Directional TOPO® Cloning, enabling you to use proofreading polymerases and to clone your PCR products in a specific orientation.
For Research Use Only. Not for use in diagnostic procedures.



Vector Type: pBAD
Cloning Method: Directional TOPO®
Promoter: araBAD
Protein Tag or Fusion: His Tag (6x), Thioredoxin, V5 Epitope Tag
Antibiotic Resistance (Bacterial): Kanamycin (KanR)
Bacterial or Yeast Strain: LMG194, TOP10
Cleavage: EK (Enterokinase) Recognition Site
Constitutive or Inducible System: Inducible
Expression: E. coli
Inducing Agent: Arabinose
Product Line: TOPO®
Product Size: 20 reactions
Selection Agent (Eukaryotic): None


Manuals & protocols